Reference - Detail
|Author||Inoue T, Ishikawa M, Sumiya Y, Kohda H, Inui T, Kuchiike D, Kubo K, Uto Y, Nishikata T.|
|Title||Establishment of a Macrophage-activating Factor Assay System Using the Human Monocytic Cell Line THP-1.|
BACKGROUND:As the mechanism of macrophage activation is not well-understood, standardization of an assay system for measuring phagocytic activities of macrophages will be useful for research on macrophages. Previously, we established a novel standardized macrophage-activating factor (MAF) assay system using U937.
MATERIALS AND METHODS:Using the human monocytic cell line THP-1, another standardized MAF assay system was established. Characteristic gene expression of U937- and THP-1-derived macrophages was compared by gene expression microarray analysis.
RESULTS:Both U937- and THP-1-derived macrophages showed obvious phagocytic activities with unique characteristics and, therefore, could not be assigned to a single sub-type.
CONCLUSION:Activation of macrophages is an intricate cellular process. Comparison of our two novel assay systems provides new insights into macrophage activation mechanisms.
|MeSH||Biological Assay / methods Cell Line Gene Expression / genetics Humans Macrophage Activation / immunology* Macrophage-Activating Factors / analysis* Macrophages / immunology* Microarray Analysis Phagocytosis / immunology* U937 Cells|
|Human and Animal Cells||THP-1(RCB1189)|