Reference - Detail
RRC ID | 37578 |
---|---|
Author | Inoue T, Ishikawa M, Sumiya Y, Kohda H, Inui T, Kuchiike D, Kubo K, Uto Y, Nishikata T. |
Title | Establishment of a Macrophage-activating Factor Assay System Using the Human Monocytic Cell Line THP-1. |
Journal | Anticancer Res |
Abstract |
BACKGROUND:As the mechanism of macrophage activation is not well-understood, standardization of an assay system for measuring phagocytic activities of macrophages will be useful for research on macrophages. Previously, we established a novel standardized macrophage-activating factor (MAF) assay system using U937. MATERIALS AND METHODS:Using the human monocytic cell line THP-1, another standardized MAF assay system was established. Characteristic gene expression of U937- and THP-1-derived macrophages was compared by gene expression microarray analysis. RESULTS:Both U937- and THP-1-derived macrophages showed obvious phagocytic activities with unique characteristics and, therefore, could not be assigned to a single sub-type. CONCLUSION:Activation of macrophages is an intricate cellular process. Comparison of our two novel assay systems provides new insights into macrophage activation mechanisms. |
Volume | 35(8) |
Pages | 4441-5 |
Published | 2015-8-1 |
PII | 35/8/4441 |
PMID | 26168484 |
MeSH | Biological Assay / methods Cell Line Gene Expression / genetics Humans Macrophage Activation / immunology* Macrophage-Activating Factors / analysis* Macrophages / immunology* Microarray Analysis Phagocytosis / immunology* U937 Cells |
IF | 1.994 |
Times Cited | 3 |
WOS Category | ONCOLOGY |
Resource | |
Human and Animal Cells | THP-1(RCB1189) |