論文 - 詳細
RRC ID | 37578 |
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著者 | Inoue T, Ishikawa M, Sumiya Y, Kohda H, Inui T, Kuchiike D, Kubo K, Uto Y, Nishikata T. |
タイトル | Establishment of a Macrophage-activating Factor Assay System Using the Human Monocytic Cell Line THP-1. |
ジャーナル | Anticancer Res |
Abstract |
BACKGROUND:As the mechanism of macrophage activation is not well-understood, standardization of an assay system for measuring phagocytic activities of macrophages will be useful for research on macrophages. Previously, we established a novel standardized macrophage-activating factor (MAF) assay system using U937. MATERIALS AND METHODS:Using the human monocytic cell line THP-1, another standardized MAF assay system was established. Characteristic gene expression of U937- and THP-1-derived macrophages was compared by gene expression microarray analysis. RESULTS:Both U937- and THP-1-derived macrophages showed obvious phagocytic activities with unique characteristics and, therefore, could not be assigned to a single sub-type. CONCLUSION:Activation of macrophages is an intricate cellular process. Comparison of our two novel assay systems provides new insights into macrophage activation mechanisms. |
巻・号 | 35(8) |
ページ | 4441-5 |
公開日 | 2015-8-1 |
PII | 35/8/4441 |
PMID | 26168484 |
MeSH | Biological Assay / methods Cell Line Gene Expression / genetics Humans Macrophage Activation / immunology* Macrophage-Activating Factors / analysis* Macrophages / immunology* Microarray Analysis Phagocytosis / immunology* U937 Cells |
IF | 1.994 |
引用数 | 3 |
WOS 分野 | ONCOLOGY |
リソース情報 | |
ヒト・動物細胞 | THP-1(RCB1189) |