論文 - 詳細
| RRC ID | 37579 |
|---|---|
| 著者 | Miura K, Nakaya H, Kobayashi Y. |
| タイトル | Experimental assessment of effects of antiproliferative drugs of drug-eluting stents on endothelial cells. |
| ジャーナル | Cardiovasc Revasc Med |
| Abstract |
BACKGROUND:Late and very late stent thrombosis after drug-eluting stent implantation is a major concern. The present study evaluated difference in the effects of sirolimus, paclitaxel and zotarolimus on endothelial cells. METHODS:Mouse endothelial cells were seeded in a 6-well plate. Cells were cultured with an antiproliferative drug at the expected concentrations for each well for 24 hours before making 3 scratch lines with a pipette tip. After a 4.5 hour incubation period, 3 reference scratch lines, vertically across the original scratch lines, were made in the same way. The experiment was repeated at least 6 times (6 plates). Measurements were performed at 9 crossings of each well. Wound healing ratio was calculated as 1-(distance of the first scratch/distance of the second scratch). % cell migration was calculated as (wound healing ratio at an expected drug concentration/wound healing ratio with no drug) × 100. Average % cell migration at 54 crossings of 6 plates was calculated. RESULTS:Paclitaxel inhibited cell migration in a concentration-dependent manner. On the other hand, concentration-dependent inhibition was not observed for sirolimus or zotarolimus. Sirolimus showed a stronger inhibitory effect on migration of endothelial cells compared to zotarolimus. CONCLUSIONS:The difference in the effect of antiproliferative drugs of drug-eluting stents on endothelial cells may be associated with relatively faster re-endothelialization of zotarolimus-eluting stent compared to the 1st generation DES. |
| 巻・号 | 16(6) |
| ページ | 344-7 |
| 公開日 | 2015-9-1 |
| DOI | 10.1016/j.carrev.2015.07.002 |
| PII | S1553-8389(15)00183-9 |
| PMID | 26253188 |
| MeSH | Animals Antineoplastic Agents / pharmacology Cell Movement / drug effects* Cells, Cultured Dose-Response Relationship, Drug Drug-Eluting Stents* Endothelial Cells / drug effects* Mice Paclitaxel / pharmacology* Sirolimus / analogs & derivatives* Sirolimus / pharmacology* |
| 引用数 | 1 |
| リソース情報 | |
| ヒト・動物細胞 | UV♀2(RCB1994) |