論文 - 詳細
| RRC ID | 37581 |
|---|---|
| 著者 | Ayabe T, Mizushige T, Ota W, Kawabata F, Hayamizu K, Han L, Tsuji T, Kanamoto R, Ohinata K. |
| タイトル | A novel Alaska pollack-derived peptide, which increases glucose uptake in skeletal muscle cells, lowers the blood glucose level in diabetic mice. |
| ジャーナル | Food Funct |
| Abstract |
We found that the tryptic digest of Alaska pollack protein exhibits a glucose-lowering effect in KK-Ay mice, a type II diabetic model. We then searched for glucose-lowering peptides in the digest. Ala-Asn-Gly-Glu-Val-Ala-Gln-Trp-Arg (ANGEVAQWR) was identified from a peak of the HPLC fraction selected based on the glucose-lowering activity in an insulin resistance test using ddY mice. ANGEVAQWR (3 mg kg(-1)) decreased the blood glucose level after intraperitoneal administration. Among its fragment peptides, the C-terminal tripeptide, Gln-Trp-Arg (QWR, 1 mg kg(-1)), lowered the blood glucose level, suggesting that the C-terminal is critical for glucose-lowering activity. QWR also enhanced glucose uptake into C2C12, a mouse skeletal muscle cell line. QWR did not induce the phosphorylation of serine/threonine protein kinase B (Akt) and adenosine monophosphate-activated protein kinase (AMPK). We also demonstrated that QWR lowered the blood glucose level in NSY and KK-Ay, type II diabetic models. |
| 巻・号 | 6(8) |
| ページ | 2749-57 |
| 公開日 | 2015-8-1 |
| DOI | 10.1039/c5fo00401b |
| PMID | 26152190 |
| MeSH | Amino Acid Sequence Animals Blood Glucose / metabolism* Diabetes Mellitus, Type 2 / drug therapy* Diabetes Mellitus, Type 2 / metabolism Fish Proteins / chemistry* Gadiformes Humans Insulin / metabolism Male Mice Molecular Sequence Data Muscle, Skeletal / drug effects Muscle, Skeletal / metabolism* Peptides / administration & dosage* Peptides / chemistry |
| IF | 4.171 |
| 引用数 | 4 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY FOOD SCIENCE & TECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |