RRC ID 37637
著者 Egusa H, Kaneda Y, Akashi Y, Hamada Y, Matsumoto T, Saeki M, Thakor DK, Tabata Y, Matsuura N, Yatani H.
タイトル Enhanced bone regeneration via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts.
ジャーナル Biomaterials
Abstract Recently, the binding sequence Ser-Val-Val-Tyr-Gly-Leu-Arg (SVVYGLR) was found adjacent to the RGD sequence in osteopontin, suggesting involvement in osteo-immune cross-talk. The aim of this study was to investigate bioactive functions of a synthetic SVVYGLR peptide in osteoprogenitor cells and osteoclasts, and to examine potential applications in bone regeneration. The SVVYGLR peptide significantly enhanced the adhesion and proliferation of several human mesenchymal cells including bone marrow-derived mesenchymal stem cells, and alphavbeta3 integrin was involved in cell attachment to the peptide. Additionally, the peptide reduced the number of TRAP-positive multinucleated cells during osteoclastogenesis of RAW264.7 cells and normal murine pre-osteoclasts, and also suppressed NFAT activity and expression of osteoclastogenesis-related mRNAs. When standardized bone defects in rat calvariae were filled with a collagen sponge containing the peptide or PBS (control), the number of TRAP-positive osteoclasts in the grafted sites after 3 weeks was significantly lower in the peptide group. By the 5th week, significantly enhanced resorption of the grafted collagen sponge and new bone formation was observed within and surrounding the sponge in the peptide group. These data suggest that SVVYGLR is an effective bioactive peptide for bone tissue regeneration that promotes attachment and proliferation of osteogenic cells while also suppressing osteoclastogenesis.
巻・号 30(27)
ページ 4676-86
公開日 2009-9-1
DOI 10.1016/j.biomaterials.2009.05.032
PII S0142-9612(09)00537-7
PMID 19520427
MeSH Adult Amino Acid Sequence Animals Bone Regeneration / drug effects* Calcification, Physiologic / drug effects Cell Adhesion / drug effects Cell Line Cell Proliferation / drug effects Humans Integrin alphaVbeta3 Male Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / drug effects Mesenchymal Stem Cells / metabolism Mice Molecular Sequence Data Osteoclasts / cytology Osteoclasts / drug effects* Osteoclasts / metabolism* Osteogenesis / drug effects Peptides / chemistry* Peptides / pharmacology* Rats Rats, Sprague-Dawley Skull / drug effects Skull / pathology Stem Cells / cytology Stem Cells / drug effects* Stem Cells / metabolism*
IF 10.317
引用数 43
WOS 分野 ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)