論文 - 詳細
| RRC ID | 37640 |
|---|---|
| 著者 | Zen K, Yasui K, Gen Y, Dohi O, Wakabayashi N, Mitsufuji S, Itoh Y, Zen Y, Nakanuma Y, Taniwaki M, Okanoue T, Yoshikawa T. |
| タイトル | Defective expression of polarity protein PAR-3 gene (PARD3) in esophageal squamous cell carcinoma. |
| ジャーナル | Oncogene |
| Abstract |
The partition-defective 3 (PAR-3) protein is implicated in the formation of tight junctions at epithelial cell-cell contacts. We investigated DNA copy number aberrations in human esophageal squamous cell carcinoma (ESCC) cell lines using a high-density oligonucleotide microarray and found a homozygous deletion of PARD3 (the gene encoding PAR-3). Exogenous expression of PARD3 in ESCC cells lacking this gene enhanced the recruitment of zonula occludens 1 (ZO-1), a marker of tight junctions, to sites of cell-cell contact. Conversely, knockdown of PARD3 in ESCC cells expressing this gene caused a disruption of ZO-1 localization at cell-cell borders. A copy number loss of PARD3 was observed in 15% of primary ESCC cells. Expression of PARD3 was significantly reduced in primary ESCC tumors compared with their nontumorous counterparts, and this reduced expression was associated with positive lymph node metastasis and poor differentiation. Our results suggest that deletion and reduced expression of PARD3 may be a novel mechanism that drives the progression of ESCC. |
| 巻・号 | 28(32) |
| ページ | 2910-8 |
| 公開日 | 2009-8-13 |
| DOI | 10.1038/onc.2009.148 |
| PII | onc2009148 |
| PMID | 19503097 |
| MeSH | Adaptor Proteins, Signal Transducing Aged Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / metabolism Carcinoma, Squamous Cell / pathology Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Cell Line, Tumor Cell Movement Esophageal Neoplasms / genetics* Esophageal Neoplasms / metabolism Esophageal Neoplasms / pathology Gene Deletion Gene Dosage Gene Expression Profiling* Gene Expression Regulation, Neoplastic* Homozygote Humans Immunoblotting Infant Intercellular Junctions / metabolism Male Membrane Proteins / genetics* Membrane Proteins / metabolism Microscopy, Confocal Microscopy, Fluorescence Middle Aged Oligonucleotide Array Sequence Analysis Phosphoproteins / metabolism RNA Interference Reverse Transcriptase Polymerase Chain Reaction Zonula Occludens-1 Protein |
| IF | 7.971 |
| 引用数 | 63 |
| WOS 分野 | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY ONCOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | TE-1(RCB1894) TE-5(RCB1949) TE-9(RCB1988) TE-15(RCB1951) EC-GI-10(RCB0774) |