| RRC ID |
37651
|
| Author |
Ishikawa H, Takeda K, Okamoto A, Matsuo S, Isobe K.
|
| Title |
Induction of autoimmunity in a bleomycin-induced murine model of experimental systemic sclerosis: an important role for CD4+ T cells.
|
| Journal |
J Invest Dermatol
|
| Abstract |
Systemic sclerosis (SSc) is an autoimmune disease characterized by the excessive deposition of collagen in the skin or other organs and the production of specific antinuclear antibodies (ANAs). Recently, bleomycin (BLM)-induced experimental scleroderma was reported in a murine model. Here, we present further development of this model and suggest that it is appropriate for the analysis of human diffuse type SSc. BLM was injected into the shaved backs of C3H or BALB/c mice (100 microg/mouse) 5 days per week for 3 weeks. Skin fibrosis was confirmed and pathological changes were seen in the lower part of the esophagus and stomach similar to those seen in SSc. The sera from these mice had autoantibodies specific to the damaged tissues and ANAs. Transfer of CD4(+) T cells from BLM-treated BALB/c mice induced the same pathological changes and antibody production in untreated-BALB/c nude mice. Hence, tissue fibrosis and the production of ANAs are probably associated with CD4(+) T-cell activity in this model. In conclusion, this model will be valuable for investigating the relationship between tissue fibrosis and abnormalities of the immune system.
|
| Volume |
129(7)
|
| Pages |
1688-95
|
| Published |
2009-7-1
|
| DOI |
10.1038/jid.2008.431
|
| PII |
S0022-202X(15)34405-5
|
| PMID |
19158840
|
| MeSH |
Adoptive Transfer
Animals
Antibiotics, Antineoplastic / toxicity
Antibodies, Antineutrophil Cytoplasmic / immunology
Autoimmunity / immunology*
Bleomycin / toxicity
CD4-Positive T-Lymphocytes / immunology*
CD4-Positive T-Lymphocytes / transplantation
Disease Models, Animal
Female
Fibrosis
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Nude
Scleroderma, Systemic / chemically induced
Scleroderma, Systemic / immunology*
Scleroderma, Systemic / pathology*
Skin / immunology
Skin / pathology
|
| IF |
7.143
|
| Times Cited |
40
|
|
WOS Category
|
DERMATOLOGY
|
| Resource |
| Human and Animal Cells |
NIH3T3 |
