| RRC ID |
37664
|
| 著者 |
Kitajima T, Sakuragi M, Hasuda H, Ozu T, Ito Y.
|
| タイトル |
A chimeric epidermal growth factor with fibrin affinity promotes repair of injured keratinocyte sheets.
|
| ジャーナル |
Acta Biomater
|
| Abstract |
The aim of the present study is to create a novel chimeric protein of epidermal growth factor (EGF) with fibrin affinity and demonstrate its potential for repairing injured tissues by immobilization to fibrin. The chimeric protein (FBD-EGF) was produced by the fusion of the fibronectin fibrin-binding domain (FBD) to EGF. It showed dose-dependent binding to fibrin and its binding was stable for at least 7days, while native EGF showed little affinity. FBD-EGF promoted the growth of fibroblasts and keratinocytes in the fibrin-bound state as well as in the soluble state. Its activity was further studied in a keratinocyte culture system in which fibrin was exposed upon injury of cell sheets. Fibrin-bound FBD-EGF promoted growth of the sheets over the injured area at a significantly faster rate (approximately eightfold) than native EGF (p<0.01). Wounds 2mm wide were closed in 7-9days. This repair process was inhibited by anti-EGF. Keratinocytes proliferated more extensively in the leading edges of sheets contacting fibrin with FBD-EGF, approximately 1.7-fold more than in the adjacent regions. These results imply that the stable binding of chimeric EGF to fibrin is effective for the repair of injured keratinocyte sheets, suggesting a potential use in tissue engineering.
|
| 巻・号 |
5(7)
|
| ページ |
2623-32
|
| 公開日 |
2009-9-1
|
| DOI |
10.1016/j.actbio.2009.03.022
|
| PII |
S1742-7061(09)00119-6
|
| PMID |
19376761
|
| MeSH |
Animals
Biocompatible Materials / chemistry
Biocompatible Materials / pharmacology
Cells, Cultured
Epidermal Growth Factor / chemistry*
Epidermal Growth Factor / pharmacology*
Fibrin / chemistry*
Keratinocytes / cytology*
Keratinocytes / drug effects
Rats
Wound Healing / drug effects*
Wound Healing / physiology
|
| IF |
7.242
|
| 引用数 |
23
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
|
