RRC ID 37724
著者 Rodríguez Hernández JC, Rico P, Moratal D, Monleón Pradas M, Salmerón-Sánchez M.
タイトル Fibrinogen patterns and activity on substrates with tailored hydroxy density.
ジャーナル Macromol Biosci
Abstract The influence of the surface fraction of OH groups on fibrinogen (FG) adsorption is investigated in copolymers of ethyl acrylate and hydroxy ethylacrylate. The amount of adsorbed FG, quantified by western-blotting combined with image analysis of the corresponding bands, decreases as the hydrophilicity of the substrate increases. The influence of substrate wettability on FG conformation and distribution is observed by atomic force microscopy (AFM). The most hydrophobic substrate promotes FG fibrillogenesis, which leads to a fibrin-like appearance in the absence of any thrombin. The degree of FG interconnection was quantified by calculating the fractal dimension of the adsorbed protein from image analysis of the AFM results. The biological activity of the adsorbed FG is correlated to cell adhesion on FG-coated substrates.
巻・号 9(8)
ページ 766-75
公開日 2009-8-11
DOI 10.1002/mabi.200800332
PMID 19422014
MeSH 3T3 Cells Acrylates / chemistry Adsorption Animals Biocompatible Materials Biomimetic Materials / chemistry* Cell Adhesion* Fibrinogen / chemistry* Hydrophobic and Hydrophilic Interactions Mice Microscopy, Atomic Force Polymers / chemistry* Surface Properties Wettability
IF 3.416
引用数 19
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)