論文 - 詳細
| RRC ID | 37726 |
|---|---|
| 著者 | Ko JA, Yanai R, Nishida T. |
| タイトル | IGF-1 released by corneal epithelial cells induces up-regulation of N-cadherin in corneal fibroblasts. |
| ジャーナル | J Cell Physiol |
| Abstract |
Interactions between epithelial cells and fibroblasts play important roles in tissue homeostasis. With the use of a coculture system in which human corneal fibroblasts and epithelial cells are cultured on opposite sides of a collagen (vitrigel) membrane, we have examined the effects of epithelial cells on expression of the adherens-junction protein N-cadherin in fibroblasts. Reverse transcription-polymerase chain reaction and immunoblot analyses showed that the presence of epithelial cells increased the expression of N-cadherin in fibroblasts at the mRNA and protein levels. This effect of epithelial cells was mimicked by insulin-like growth factor-1 (IGF-1) but not by epidermal growth factor or fibroblast growth factor. Depletion of IGF-1 in epithelial cells by RNA interference abolished the effect of these cells on N-cadherin expression in fibroblasts. The presence of epithelial cells activated the IGF-1 receptor as well as up-regulated expression of the transcriptional regulator ZEB1 in fibroblasts. RNA interference-mediated depletion of IGF-1 in epithelial cells prevented the effect of these cells on ZEB1 expression in fibroblasts. These results suggest that IGF-1 released from corneal epithelial cells up-regulates the expression of N-cadherin in corneal fibroblasts. |
| 巻・号 | 221(1) |
| ページ | 254-61 |
| 公開日 | 2009-10-1 |
| DOI | 10.1002/jcp.21850 |
| PMID | 19530221 |
| MeSH | Adolescent Adult Aged Antigens, CD / metabolism* Cadherins / metabolism* Child Child, Preschool Coculture Techniques Epithelial Cells / cytology Epithelial Cells / drug effects Epithelium, Corneal / cytology* Epithelium, Corneal / metabolism* Female Fibroblasts / drug effects Fibroblasts / metabolism* Homeodomain Proteins / metabolism Humans Insulin-Like Growth Factor I / metabolism* Intercellular Signaling Peptides and Proteins / pharmacology Male Membrane Proteins / metabolism Middle Aged Phosphorylation / drug effects RNA Interference / drug effects Signal Transduction / drug effects Tight Junctions / drug effects Tight Junctions / metabolism Time Factors Transcription Factors / metabolism Up-Regulation* / drug effects Zinc Finger E-box-Binding Homeobox 1 |
| IF | 5.546 |
| 引用数 | 15 |
| WOS 分野 | PHYSIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |