| RRC ID |
37736
|
| Author |
Inafuku Y, Furuhata T, Tayama M, Okita K, Nishidate T, Mizuguchi T, Kimura Y, Hirata K.
|
| Title |
Matrix metalloproteinase-2 expression in stromal tissues is a consistent prognostic factor in stage II colon cancer.
|
| Journal |
Cancer Sci
|
| Abstract |
For patients with stage II colon cancer, the usefulness of adjuvant chemotherapy remains controversial. Therefore, it is important to identify high-risk indicators. The biological prognostic factors for recurrence might allow further insight into the optimal treatment strategy for patients with node-negative disease. Matrix metalloproteinase-2 seems to be one of the essential factors for tumor invasion and lymph node metastasis. In this study, we analyzed the expression of cyclooxygenase-2 and matrix metalloproteinase-2 by immunohistochemical staining in 109 patients with stage II colon cancer. A positive correlation was observed between tumor cyclooxygenase-2 and tumor matrix metalloproteinase-2 expression (P = 0.0006) and between tumor cyclooxygenase-2 and stromal matrix metalloproteinase-2 expression (P < 0.0001). Stromal matrix metalloproteinase-2 expression was associated with disease-free survival (P = 0.0095) and was shown to be an independent risk factor for recurrence by multivariate analysis. In addition, we carried out an invasion assay in vitro to investigate whether cyclooxygenase-2 and matrix metalloproteinase-2 affected the tumor-invasive potential of colon cancer cell lines. The invasion assay showed that every cancer cell line acquired invasive potential in coculture with stromal cell lines and the cyclooxygenase-2 inhibitor suppressed this phenomenon by downregulating the matrix metalloproteinase-2 expression of stromal cells. In conclusion, these findings suggest that matrix metalloproteinase-2 expression in stromal cells can be a high-risk indicator for recurrence in patients with stage II colon cancer.
|
| Volume |
100(5)
|
| Pages |
852-8
|
| Published |
2009-5-1
|
| DOI |
10.1111/j.1349-7006.2009.01116.x
|
| PMID |
19445018
|
| MeSH |
Cell Line
Colonic Neoplasms / enzymology*
Colonic Neoplasms / pathology*
Cyclooxygenase 2 / metabolism
Cyclooxygenase 2 Inhibitors / pharmacology
Female
Gastrointestinal Stromal Tumors / enzymology*
Gastrointestinal Stromal Tumors / pathology*
Gene Expression Regulation, Neoplastic / genetics
Humans
Male
Matrix Metalloproteinase 2 / metabolism*
Middle Aged
Neoplasm Invasiveness / pathology
Neoplasm Staging
Prognosis
Survival Rate
|
| IF |
4.966
|
| Times Cited |
11
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
THP-1(RCB1189) |
