Reference - Detail
| RRC ID | 37737 |
|---|---|
| Author | Tsunoda N, Kokuryo T, Oda K, Senga T, Yokoyama Y, Nagino M, Nimura Y, Hamaguchi M. |
| Title | Nek2 as a novel molecular target for the treatment of breast carcinoma. |
| Journal | Cancer Sci |
| Abstract |
We investigated the role of Nek2, a member of the serine-threonine kinase family, in the tumorigenic growth of breast carcinoma. Increased expression of Nek2 was observed in all breast carcinoma cell lines examined (BT20, BT474, Hs578T, MCF7, MDA-MB-231, T47D, and ZR-75-1) by immunoblotting. By treatment with Nek2 short interfering RNA (siRNA), expression of Nek2 was clearly decreased in both estrogen receptor (ER)-positive (MCF7) and ER-negative (MDA-MB-231) breast carcinoma cell lines. Cell growth, colony formation in soft agar, and in vitro invasiveness of these cell lines were substantially suppressed by Nek2 siRNA treatment. In a xenograft nude mouse model with subcutaneous implantation of MCF7 or MDA-MB-231, subcutaneous injection of Nek2 siRNA around the tumor nodules resulted in a reduction of tumor size compared with those of control siRNA injection. Taken together, Nek2 appears to play a pivotal role in tumorigenic growth of breast carcinoma cells, and could be a useful therapeutic molecular target for the treatment of breast carcinoma both in ER-positive and ER-negative cases. |
| Volume | 100(1) |
| Pages | 111-6 |
| Published | 2009-1-1 |
| DOI | 10.1111/j.1349-7006.2008.01007.x |
| PII | CAS1007 |
| PMID | 19038001 |
| MeSH | Animals Breast Neoplasms / chemistry Breast Neoplasms / pathology Breast Neoplasms / therapy* Cell Line, Tumor Female Humans Male Mice NIMA-Related Kinases Protein Serine-Threonine Kinases / antagonists & inhibitors* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / physiology RNA, Small Interfering / genetics Receptors, Estrogen / analysis |
| IF | 4.966 |
| Times Cited | 77 |
| WOS Category | ONCOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | |