RRC ID 37753
Author Kawahara Y, Manabe T, Matsumoto M, Kajiume T, Matsumoto M, Yuge L.
Title LIF-free embryonic stem cell culture in simulated microgravity.
Journal PLoS One
Abstract BACKGROUND:Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required.
METHODOLOGY/PRINCIPAL FINDINGS:We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF.
CONCLUSIONS/SIGNIFICANCE:Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells.
Volume 4(7)
Pages e6343
Published 2009-7-23
DOI 10.1371/journal.pone.0006343
PMID 19626124
PMC PMC2710515
MeSH Animals Base Sequence Cell Culture Techniques DNA Primers Embryonic Stem Cells / cytology* Leukemia Inhibitory Factor / metabolism* Mice Polymerase Chain Reaction Weightlessness*
IF 2.74
Times Cited 30
Human and Animal Cells BRC6(AES0010)