RRC ID 37778
著者 Choi SS, Cha BY, Lee YS, Yonezawa T, Teruya T, Nagai K, Woo JT.
タイトル Magnolol enhances adipocyte differentiation and glucose uptake in 3T3-L1 cells.
ジャーナル Life Sci
Abstract AIMS:The nuclear receptor peroxisome proliferator-activated receptor (PPAR) gamma plays an important role in adipocyte differentiation. Its ligands, including thiazolidinediones, improve insulin sensitivity in type 2 diabetes. We investigate the effect of magnolol, an ingredient of Magnolia officinalis on adipogenesis and glucose uptake using 3T3-L1 cells.
MAIN METHODS:The effect of magnolol on adipocyte differentiation was quantified by measuring Oil Rd O staining using 3T3-L1 cells and C3H10T1/2 cells. And real-time PCR and western blot were used to determine the expression of PPARgamma or PPARgamma target genes, respectively. The effect of magnolol on glucose uptake was performed using 3T3-L1 adipocytes.
KEY FINDINGS:Magnolol dose-dependently enhanced adipocyte differentiation in 3T3-L1 cells and C3H10T1/2 cells. In the early stage of adipogenesis, magnolol induced gene expression of C/EBPdelta, C/EBPalpha and PPARgamma2 and during adipocyte differentiation, it also induced the expression of PPARgamma target genes such as aP2, LPL and adiponectin. In addition, magnolol it also increase expression of PAPRgamma target gene such as C/EBPalpha and aP2 at mRNA and aP2 protein level in mature adipocytes. In PPARgamma ligand binding assays, magnolol exhibited binding affinity to PPARgamma but its activity was weaker than rosiglitazone. At the same time, magnolol-induced adipogenesis was inhibited by co-treatment of GW9662 both 3T3-L1 cells and C3H10T1/2 cells. In mature 3T3-L1 adipocytes, magnolol increased basal and insulin-stimulated glucose uptake accompanied by the up-regulation of mRNA and protein level of Glut4.
SIGNIFICANCE:Our results suggest that magnolol could improve insulin sensitivity through the activation of PPARgamma as a ligand.
巻・号 84(25-26)
ページ 908-14
公開日 2009-6-19
DOI 10.1016/j.lfs.2009.04.001
PII S0024-3205(09)00158-1
PMID 19376135
MeSH 3T3-L1 Cells / cytology 3T3-L1 Cells / metabolism Adipocytes / cytology* Adipocytes / metabolism Animals Biphenyl Compounds / metabolism Biphenyl Compounds / pharmacology* Cell Differentiation / drug effects Cell Membrane Permeability / drug effects Gene Expression Regulation Glucose / metabolism* Insulin / metabolism Ligands Lignans / metabolism Lignans / pharmacology* Magnolia / chemistry Mice Peroxisome Proliferator-Activated Receptors / genetics Peroxisome Proliferator-Activated Receptors / metabolism* Pluripotent Stem Cells / cytology Pluripotent Stem Cells / metabolism Protein Binding RNA, Messenger / genetics
IF 3.647
引用数 80
WOS 分野 PHARMACOLOGY & PHARMACY MEDICINE, RESEARCH & EXPERIMENTAL
リソース情報
ヒト・動物細胞 10T1/2(RCB0247)