RRC ID 37779
Author Iwasaki Y, Tanabe M, Kayama Y, Abe M, Kashio M, Koizumi K, Okumura Y, Morimitsu Y, Tominaga M, Ozawa Y, Watanabe T.
Title Miogadial and miogatrial with alpha,beta-unsaturated 1,4-dialdehyde moieties--novel and potent TRPA1 agonists.
Journal Life Sci.
Abstract AIMS:Most of the terpenoids with an alpha,beta-unsaturated 1,4-dialdehyde moiety, which are found in plants, fungi, and insects, have a pungent taste. However, the neural receptors responsible for the pungency of these terpenoids have not been identified yet. The transient receptor potential ankyrin 1 (TRPA1) and transient receptor potential vanilloid 1 (TRPV1), which are expressed in the nociceptive neurons, induce a sensation of heat on activation by some pungent ingredients in food. In this study, we selected miogadial (MD), miogatrial (MT), and polygodial (PG) from the terpenoids with an alpha,beta-unsaturated 1,4-dialdehyde moiety and examined the effects of these 3 terpenoids on TRPA1 or TRPV1.
MAIN METHODS:TRPV1 and TRPA1 activity by 3 terpenoids were evaluated using Ca(2+) imaging and patch-clamp methods in mammalian cells that express TRP heterologously and mouse sensory neurons.
KEY FINDINGS:The 3 terpenoids activated TRPA1 that was heterologously expressed in HEK293 or CHO cells. The potencies of activation by the 3 terpenoids were equal and almost 10 times stronger than that of allyl isothiocyanate (AITC), which is known as the most potent TRPA1 agonist among all natural products. Moreover, these 3 terpenoids exhibited increased intracellular Ca(2+) concentration in mouse sensory neuron cells compared to AITC. High concentrations of the 3 terpenoids also activated TRPV1 that was heterologously expressed in HEK293 cells.
SIGNIFICANCE:These results indicated that MD, MT, and PG were more potent in activating TRPA1 than TRPV1, and suggested that they primarily activate TRPA1 to induce pungency.
Volume 85(1-2)
Pages 60-9
Published 2009-7-3
DOI 10.1016/j.lfs.2009.04.017
PII S0024-3205(09)00185-4
PMID 19409911
MeSH Aldehydes / chemistry* Animals Behavior, Animal / drug effects CHO Cells Calcium / metabolism Cell Line Cricetinae Cricetulus Diterpenes / chemistry* Diterpenes / pharmacology* Electrophysiology Ganglia, Spinal / cytology Ganglia, Spinal / drug effects Humans Male Mice Mice, Inbred C57BL Pain / psychology Sensory Receptor Cells / drug effects Sesquiterpenes / pharmacology Structure-Activity Relationship TRPA1 Cation Channel TRPV Cation Channels / agonists Transient Receptor Potential Channels / agonists*
IF 3.234
Times Cited 28
Human and Animal Cells