論文 - 詳細
| RRC ID | 37781 |
|---|---|
| 著者 | Fusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M. |
| タイトル | Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome. |
| ジャーナル | Proc Jpn Acad Ser B Phys Biol Sci |
| Abstract |
Induced pluripotent stem cells (iPSC) have been generated from somatic cells by introducing reprogramming factors. Integration of foreign genes into the host genome is a technical hurdle for the clinical application. Here, we show that Sendai virus (SeV), an RNA virus and carries no risk of altering host genome, is an efficient solution for generating safe iPSC. Sendai-viral human iPSC expressed pluripotency genes, showed demethylation characteristic of reprogrammed cells. SeV-derived transgenes were decreased during cell division. Moreover, viruses were able to be easily removed by antibody-mediated negative selection utilizing cell surface marker HN that is expressed on SeV-infected cells. Viral-free iPSC differentiated to mature cells of the three embryonic germ layers in vivo and in vitro including beating cardiomyocytes, neurons, bone and pancreatic cells. Our data demonstrated that highly-efficient, non-integrating SeV-based vector system provides a critical solution for reprogramming somatic cells and will accelerate the clinical application. |
| 巻・号 | 85(8) |
| ページ | 348-62 |
| 公開日 | 2009-1-1 |
| DOI | 10.2183/pjab.85.348 |
| PII | JST.JSTAGE/pjab/85.348 |
| PMID | 19838014 |
| PMC | PMC3621571 |
| MeSH | Biomarkers / metabolism Cell Line, Tumor Cell Proliferation Cellular Reprogramming / genetics* Cellular Reprogramming / physiology DNA Methylation Fibroblasts / metabolism Gene Expression Profiling Genetic Vectors / genetics* Genetic Vectors / isolation & purification Genome, Human / genetics* Humans Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism* Sendai virus / genetics* Sendai virus / isolation & purification Transduction, Genetic Transgenes / genetics* Virus Integration |
| IF | 3.0 |
| 引用数 | 760 |
| WOS 分野 | MULTIDISCIPLINARY SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 215 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-G2/PA6(RCB1127) |