| RRC ID |
37785
|
| 著者 |
Nishida T, Ito J, Nagayasu Y, Yokoyama S.
|
| タイトル |
FGF-1-induced reactions for biogenesis of apoE-HDL are mediated by src in rat astrocytes.
|
| ジャーナル |
J Biochem
|
| Abstract |
Fibroblast growth factor-1 (FGF-1) is released from astrocytes in stress and stimulates MEK/ERK and PI3K/Akt pathways in autocrine fashion to increase synthesis of cholesterol and 25-OH-cholesterol, and to induce transport and secretion of apoE, respectively. FGF-1-induced phosphorylation of Src, and phosphorylation of MEK, ERK and Ark was inhibited by Src inhibitors in rat astrocytes. Src inhibitors also suppressed FGF-1-induced increase of biosynthesis and release of cholesterol and increase of apolipoprotein E (apoE) secretion. The results were reproduced in rat astrocytoma cells transfected by rat apoE and in 3T3-L1 cells. Down-regulation of Src expression reduced FGF-1-induced phosphorylation of the signalling protein and subsequent reactions. Increase by FGF-1 of messages of apoE and HMG-CoA reductase was not influenced by Src inhibitors or by its down-regulation. We conclude that FGF-1 activates Src for activation of MEK/ERK and PI3K/Akt pathways, while Src may not be involved in enhancement of transcription of the cholesterol-related genes.
|
| 巻・号 |
146(6)
|
| ページ |
881-6
|
| 公開日 |
2009-12-1
|
| DOI |
10.1093/jb/mvp135
|
| PII |
mvp135
|
| PMID |
19713443
|
| MeSH |
Animals
Apolipoproteins E / metabolism*
Astrocytes / metabolism*
Brain / cytology
Cells, Cultured
Embryo, Mammalian
Female
Fibroblast Growth Factor 1 / metabolism*
Lipoproteins, HDL / metabolism*
Models, Biological
RNA, Small Interfering
Rats
Rats, Wistar
Signal Transduction
src-Family Kinases / metabolism*
|
| IF |
2.476
|
| 引用数 |
5
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
