論文 - 詳細
| RRC ID | 37806 |
|---|---|
| 著者 | Peng TL, Chen J, Mao W, Liu X, Tao Y, Chen LZ, Chen MH. |
| タイトル | Potential therapeutic significance of increased expression of aryl hydrocarbon receptor in human gastric cancer. |
| ジャーナル | World J Gastroenterol |
| Abstract |
AIM:To determine the functional significance of aryl hydrocarbon receptor (AhR) in gastric carcinogenesis, and to explore the possible role of AhR in gastric cancer (GC) treatment. METHODS:RT-PCR, real-time PCR, and Western blotting were performed to detect AhR expression in 39 GC tissues and five GC cell lines. AhR protein was detected by immunohistochemistry (IHC) in 190 samples: 30 chronic superficial gastritis (CSG), 30 chronic atrophic gastritis (CAG), 30 intestinal metaplasia (IM), 30 atypical hyperplasia (AH), and 70 GC. The AhR agonist tetrachlorodibenzo-para-dioxin (TCDD) was used to treat AGS cells. MTT assay and flow cytometric analysis were performed to measure the viability, cell cycle and apoptosis of AGS cells. RESULTS:AhR expression was significantly increased in GC tissues and GC cell lines. IHC results indicated that the levels of AhR expression gradually increased, with the lowest levels in CSG, followed by CAG, IM, AH and GC. AhR expression and nuclear translocation were significantly higher in GC than in precancerous tissues. TCDD inhibited proliferation of AGS cells via induction of growth arrest at the G1-S phase. CONCLUSION:AhR plays an important role in gastric carcinogenesis. AhR may be a potential therapeutic target for GC treatment. |
| 巻・号 | 15(14) |
| ページ | 1719-29 |
| 公開日 | 2009-4-14 |
| DOI | 10.3748/wjg.15.1719 |
| PMID | 19360915 |
| PMC | PMC2668777 |
| MeSH | Adult Aged Apoptosis / drug effects Cell Cycle / drug effects Cell Line, Tumor Environmental Pollutants / pharmacology Female Gastric Mucosa / metabolism Humans Male Microarray Analysis Middle Aged Polychlorinated Dibenzodioxins / pharmacology Precancerous Conditions / genetics Precancerous Conditions / metabolism Precancerous Conditions / pathology Receptors, Aryl Hydrocarbon / genetics Receptors, Aryl Hydrocarbon / metabolism* Signal Transduction / physiology Stomach / cytology Stomach / pathology Stomach Neoplasms / genetics Stomach Neoplasms / metabolism* Stomach Neoplasms / pathology* Stomach Neoplasms / therapy |
| IF | 3.665 |
| 引用数 | 21 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |