| RRC ID |
37815
|
| 著者 |
Hisatsune A, Kawasaki M, Nakayama H, Mikami Y, Miyata T, Isohama Y, Katsuki H, Kim KC.
|
| タイトル |
Internalization of MUC1 by anti-MUC1 antibody from cell membrane through the macropinocytotic pathway.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
MUC1 is a type I transmembrane glycoprotein aberrantly overexpressed in various cancer cells. It is thought to serve as a physical barrier from the extracellular environment and also as a receptor for various extracellular molecules. However, little is known about the fate of MUC1 during and after the interaction with these molecules. In the present study, we used anti-MUC1 antibody as an interacting molecule and investigated the cellular trafficking of MUC1. Our results showed that: (1) anti-MUC1 antibody was internalized only in MUC1 expressing cells and triggered internalization and down-regulation of MUC1; (2) the internalization of MUC1 by anti-MUC1 antibody required the cytoplasmic tail of MUC1 and was suppressed by inhibitors of Na(+)/H(+) exchanger, and caveola/raft-dependent internalization, but not by an inhibitor of clathrin-dependent internalization. We conclude that antibody-induced internalization of MUC1 involves the macropinocytotic pathway.
|
| 巻・号 |
388(4)
|
| ページ |
677-82
|
| 公開日 |
2009-10-30
|
| DOI |
10.1016/j.bbrc.2009.08.059
|
| PII |
S0006-291X(09)01628-3
|
| PMID |
19683510
|
| MeSH |
Antibodies, Monoclonal / immunology*
Cell Line
Cell Membrane / metabolism*
Humans
Mucin-1 / genetics
Mucin-1 / immunology
Mucin-1 / metabolism*
Pinocytosis*
Protein Transport
|
| IF |
2.985
|
| 引用数 |
7
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
