論文 - 詳細
| RRC ID | 37815 |
|---|---|
| 著者 | Hisatsune A, Kawasaki M, Nakayama H, Mikami Y, Miyata T, Isohama Y, Katsuki H, Kim KC. |
| タイトル | Internalization of MUC1 by anti-MUC1 antibody from cell membrane through the macropinocytotic pathway. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
MUC1 is a type I transmembrane glycoprotein aberrantly overexpressed in various cancer cells. It is thought to serve as a physical barrier from the extracellular environment and also as a receptor for various extracellular molecules. However, little is known about the fate of MUC1 during and after the interaction with these molecules. In the present study, we used anti-MUC1 antibody as an interacting molecule and investigated the cellular trafficking of MUC1. Our results showed that: (1) anti-MUC1 antibody was internalized only in MUC1 expressing cells and triggered internalization and down-regulation of MUC1; (2) the internalization of MUC1 by anti-MUC1 antibody required the cytoplasmic tail of MUC1 and was suppressed by inhibitors of Na(+)/H(+) exchanger, and caveola/raft-dependent internalization, but not by an inhibitor of clathrin-dependent internalization. We conclude that antibody-induced internalization of MUC1 involves the macropinocytotic pathway. |
| 巻・号 | 388(4) |
| ページ | 677-82 |
| 公開日 | 2009-10-30 |
| DOI | 10.1016/j.bbrc.2009.08.059 |
| PII | S0006-291X(09)01628-3 |
| PMID | 19683510 |
| MeSH | Antibodies, Monoclonal / immunology* Cell Line Cell Membrane / metabolism* Humans Mucin-1 / genetics Mucin-1 / immunology Mucin-1 / metabolism* Pinocytosis* Protein Transport |
| IF | 2.985 |
| 引用数 | 7 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | |