Reference - Detail
| RRC ID | 37817 |
|---|---|
| Author | Horie I, Maeda M, Yokoyama S, Hisatsune A, Katsuki H, Miyata T, Isohama Y. |
| Title | Tumor necrosis factor-alpha decreases aquaporin-3 expression in DJM-1 keratinocytes. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Aquaporin-3 (AQP3) is a water/glycerol-transporting protein that is strongly expressed at the plasma membranes of keratinocytes in skin. There is evidence for involvement of AQP3-facilitated water and glycerol transport in skin hydration and wound repair, respectively. In this study, we show that tumor necrosis factor-alpha (TNF-alpha) and TNF receptor-1 signaling decreased AQP3 protein expression and plasma membrane water permeability in DJM-1 keratinocytes. TNF-alpha also decreased AQP3 mRNA expression and promoter activity, indicating that TNF-alpha suppresses AQP3 gene transcription. In addition, inhibitors of p38 and extracellular signal-regulated kinase (ERK) abolished the effect of TNF-alpha on AQP3 expression level, whereas inhibitors for NF-kappaB did not. These data indicate that TNF-alpha decreases AQP3 gene expression through p38 and ERK activation, and suggest that the decrease in AQP3 expression caused by TNF-alpha might be related to the phenotypes of skin inflammation, such as dry skin. |
| Volume | 387(3) |
| Pages | 564-8 |
| Published | 2009-9-25 |
| DOI | 10.1016/j.bbrc.2009.07.077 |
| PII | S0006-291X(09)01413-2 |
| PMID | 19619514 |
| MeSH | Aquaporin 3 / biosynthesis* Aquaporin 3 / genetics Cell Line Extracellular Signal-Regulated MAP Kinases / metabolism Gene Expression Regulation* Glycerol / metabolism Humans Keratinocytes / drug effects Keratinocytes / metabolism* Promoter Regions, Genetic RNA Stability RNA, Messenger / biosynthesis Transcription, Genetic Tumor Necrosis Factor-alpha / metabolism* Tumor Necrosis Factor-alpha / pharmacology Water / metabolism p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 2.985 |
| Times Cited | 24 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Wikipedia |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | DJM-1(RCB0736) |