| RRC ID |
37817
|
| Author |
Horie I, Maeda M, Yokoyama S, Hisatsune A, Katsuki H, Miyata T, Isohama Y.
|
| Title |
Tumor necrosis factor-alpha decreases aquaporin-3 expression in DJM-1 keratinocytes.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Aquaporin-3 (AQP3) is a water/glycerol-transporting protein that is strongly expressed at the plasma membranes of keratinocytes in skin. There is evidence for involvement of AQP3-facilitated water and glycerol transport in skin hydration and wound repair, respectively. In this study, we show that tumor necrosis factor-alpha (TNF-alpha) and TNF receptor-1 signaling decreased AQP3 protein expression and plasma membrane water permeability in DJM-1 keratinocytes. TNF-alpha also decreased AQP3 mRNA expression and promoter activity, indicating that TNF-alpha suppresses AQP3 gene transcription. In addition, inhibitors of p38 and extracellular signal-regulated kinase (ERK) abolished the effect of TNF-alpha on AQP3 expression level, whereas inhibitors for NF-kappaB did not. These data indicate that TNF-alpha decreases AQP3 gene expression through p38 and ERK activation, and suggest that the decrease in AQP3 expression caused by TNF-alpha might be related to the phenotypes of skin inflammation, such as dry skin.
|
| Volume |
387(3)
|
| Pages |
564-8
|
| Published |
2009-9-25
|
| DOI |
10.1016/j.bbrc.2009.07.077
|
| PII |
S0006-291X(09)01413-2
|
| PMID |
19619514
|
| MeSH |
Aquaporin 3 / biosynthesis*
Aquaporin 3 / genetics
Cell Line
Extracellular Signal-Regulated MAP Kinases / metabolism
Gene Expression Regulation*
Glycerol / metabolism
Humans
Keratinocytes / drug effects
Keratinocytes / metabolism*
Promoter Regions, Genetic
RNA Stability
RNA, Messenger / biosynthesis
Transcription, Genetic
Tumor Necrosis Factor-alpha / metabolism*
Tumor Necrosis Factor-alpha / pharmacology
Water / metabolism
p38 Mitogen-Activated Protein Kinases / metabolism
|
| IF |
2.985
|
| Times Cited |
24
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
