Reference - Detail
| RRC ID | 37832 |
|---|---|
| Author | Tanaka M, Yamazaki M, Chiba K. |
| Title | Neuroprotective action of genipin on tunicamycin-induced cytotoxicity in neuro2a cells. |
| Journal | Biol Pharm Bull |
| Abstract |
Accumulation of unfolding or misfolded proteins within the lumen of the endoplasmic reticulum (ER) triggers ER stress, and sustained ER stress ultimately leads to cell death. Both of these events are involved in the activation of glucose-regulated protein of 78 kDa (GRP78, also known as Bip), CCAAT/enhancer-binding protein (C/EBP) homologous protein (CHOP, also known as growth arrest and DNA damage-inducible gene 153 (GADD153)), and caspase-12. ER stress has been shown to be involved in neurodegenerative disorders, such as Alzheimer, Parkinson, and polyglutamine diseases. We previously showed that genipin, a natural iridoid compound, has a protective effect against amyloid-beta (Abeta)-induced cytotoxicity. Here, we studied the protective effects of genipin on cytotoxicity induced in Neuro2a cells by the specific ER stress inducer tunicamycin (TM). TM treatment significantly reduced cell viability in a dose-dependent manner. Genipin dramatically rescued the cells against TM-induced cell death. In addition, genipin suppressed ER stress-induced upregulation of CHOP and GRP78. These data suggest that genipin is effective at protecting against neurodegenerative disorders. |
| Volume | 32(7) |
| Pages | 1220-3 |
| Published | 2009-7-1 |
| DOI | 10.1248/bpb.32.1220 |
| PII | JST.JSTAGE/bpb/32.1220 |
| PMID | 19571389 |
| MeSH | Animals Blotting, Western CCAAT-Enhancer-Binding Proteins / metabolism Caspase 12 / metabolism Cell Line, Tumor Cell Survival / drug effects Dose-Response Relationship, Drug Endoplasmic Reticulum / drug effects Endoplasmic Reticulum / metabolism Endoplasmic Reticulum Chaperone BiP Heat-Shock Proteins / metabolism Iridoid Glycosides Iridoids / pharmacology* Mice Neuroprotective Agents / pharmacology* Protein Folding Reverse Transcriptase Polymerase Chain Reaction Transcription Factor CHOP / metabolism Tunicamycin / toxicity* |
| IF | 1.863 |
| Times Cited | 30 |
| WOS Category | PHARMACOLOGY & PHARMACY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | |