| RRC ID |
37845
|
| 著者 |
Itoh M, Abe Y, Iwama Y, Kimura F, Satoh M, Shoji M, Takahashi J, Toshima G, Sasaki H, Hiwatashi K, Hata K.
|
| タイトル |
HPLC analysis of lipoproteins in culture medium of hepatoma cells: an in vitro system for screening antihyperlipidemic drugs.
|
| ジャーナル |
Biotechnol Lett
|
| Abstract |
We isolated a HepG2-derived sub-clone (HepG2-Lipo), which possessed an increased lipoprotein synthesizing ability. HepG2-Lipo cells could secrete triglycerides (TG) and cholesterol at rates 9.4- and 6-fold higher, respectively, when compared to HepG2 cells. Real-time RT-PCR analysis revealed that the expression levels of sterol regulatory element-binding protein-1c and -2 were 2.9- and 1.5-fold higher than in HepG2 cells. Furthermore, two apolipoprotein (apo) genes (apoA-1 and apoB-100) in HepG2-Lipo cells were expressed at 2.8- and 1.9-fold higher levels when compared to those in parental cells. We examined the effects of three antihyperlipidemic agents on the lipoprotein profiles of HepG2-Lipo cells. Simvastatin at 5 microM selectively suppressed cholesterol secretion from HepG2-Lipo cells, and 500 microM fenofibrate inhibited both TG and cholesterol secretion from the cells.
|
| 巻・号 |
31(7)
|
| ページ |
953-7
|
| 公開日 |
2009-7-1
|
| DOI |
10.1007/s10529-009-9978-4
|
| PMID |
19330486
|
| MeSH |
Apolipoprotein A-I / biosynthesis
Apolipoprotein B-100 / biosynthesis
Cell Line, Tumor
Chromatography, High Pressure Liquid
Culture Media / chemistry*
Fenofibrate / pharmacology
Gene Expression Profiling
Hepatocytes / drug effects*
Hepatocytes / metabolism*
Humans
Hypolipidemic Agents / pharmacology*
Lipoproteins / analysis*
Simvastatin / pharmacology
Sterol Regulatory Element Binding Protein 1 / biosynthesis
Sterol Regulatory Element Binding Protein 2 / biosynthesis
|
| IF |
1.977
|
| 引用数 |
7
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
