| RRC ID |
37846
|
| 著者 |
Felipe Alves CA, Ishikawa H, Ikeda M, Kobayashi M.
|
| タイトル |
hycu-hr6, A large homologous region of the Hyphantria cunea nucleopolyhedrovirus genome, as a powerful and versatile enhancer in insect expression systems.
|
| ジャーナル |
Virus Genes
|
| Abstract |
We previously identified a large homologous region (hr), hycu-hr6, in the genome of the Hyphantria cunea nucleopolyhedrovirus (HycuNPV) and suggested that hycu-hr6 was the largest baculovirus promoter enhancer hr identified so far. In this study, we examined the enhancement activity of hycu-hr6 against two promoters from constitutive baculovirus immediate early genes, the HycuNPV ie1 (hycu-ie1) and the Orgyia pseudotsugata multicapsid (M) NPV ie2 (op-ie2), and against a promoter from the inducible Drosophila heat shock protein 70 gene (hsp70) in five lepidopteran (BmN-4, Ld652Y, Sf9, SpIm, and TN368) and one dipteran (S2) insect cell lines. Comparative analyses of transient expression assays using the firefly luciferase gene (luc) as a reporter showed that hycu-hr6 enhanced the activity of all three promoters in all tested cell lines. Comparison of the enhancement efficiency of hycu-hr6 with that of Autographa californica MNPV (AcMNPV) hr5, an enhancer commonly used to improve the activity of AcMNPV ie1 promoter in many insect expression vectors, established that hycu-hr6 was a more efficient enhancer. These results indicate that hycu-hr6 is a versatile and superior promoter enhancer in several insect cell lines and favor the incorporation of hycu-hr6 into insect expression vectors to maximize promoter activity.
|
| 巻・号 |
39(3)
|
| ページ |
403-8
|
| 公開日 |
2009-12-1
|
| DOI |
10.1007/s11262-009-0406-6
|
| PMID |
19809870
|
| MeSH |
Animals
Cell Line
Enhancer Elements, Genetic*
Gene Expression*
Genes, Reporter
Genetic Engineering / methods*
Insecta
Luciferases, Firefly / biosynthesis
Luciferases, Firefly / genetics
Nucleopolyhedroviruses / genetics*
Promoter Regions, Genetic*
|
| IF |
1.991
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
|
