論文 - 詳細
| RRC ID | 37846 |
|---|---|
| 著者 | Felipe Alves CA, Ishikawa H, Ikeda M, Kobayashi M. |
| タイトル | hycu-hr6, A large homologous region of the Hyphantria cunea nucleopolyhedrovirus genome, as a powerful and versatile enhancer in insect expression systems. |
| ジャーナル | Virus Genes |
| Abstract |
We previously identified a large homologous region (hr), hycu-hr6, in the genome of the Hyphantria cunea nucleopolyhedrovirus (HycuNPV) and suggested that hycu-hr6 was the largest baculovirus promoter enhancer hr identified so far. In this study, we examined the enhancement activity of hycu-hr6 against two promoters from constitutive baculovirus immediate early genes, the HycuNPV ie1 (hycu-ie1) and the Orgyia pseudotsugata multicapsid (M) NPV ie2 (op-ie2), and against a promoter from the inducible Drosophila heat shock protein 70 gene (hsp70) in five lepidopteran (BmN-4, Ld652Y, Sf9, SpIm, and TN368) and one dipteran (S2) insect cell lines. Comparative analyses of transient expression assays using the firefly luciferase gene (luc) as a reporter showed that hycu-hr6 enhanced the activity of all three promoters in all tested cell lines. Comparison of the enhancement efficiency of hycu-hr6 with that of Autographa californica MNPV (AcMNPV) hr5, an enhancer commonly used to improve the activity of AcMNPV ie1 promoter in many insect expression vectors, established that hycu-hr6 was a more efficient enhancer. These results indicate that hycu-hr6 is a versatile and superior promoter enhancer in several insect cell lines and favor the incorporation of hycu-hr6 into insect expression vectors to maximize promoter activity. |
| 巻・号 | 39(3) |
| ページ | 403-8 |
| 公開日 | 2009-12-1 |
| DOI | 10.1007/s11262-009-0406-6 |
| PMID | 19809870 |
| MeSH | Animals Cell Line Enhancer Elements, Genetic* Gene Expression* Genes, Reporter Genetic Engineering / methods* Insecta Luciferases, Firefly / biosynthesis Luciferases, Firefly / genetics Nucleopolyhedroviruses / genetics* Promoter Regions, Genetic* |
| IF | 1.991 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |