論文 - 詳細
| RRC ID | 37854 |
|---|---|
| 著者 | Kobayashi H, Nishikawa M, Sakamoto C, Nishio T, Kanazawa H, Okano T. |
| タイトル | Dual temperature- and pH-responsive fluorescence molecular probe for cellular imaging utilizing a PNIPAAm-fluorescein copolymer. |
| ジャーナル | Anal Sci |
| Abstract |
The thermally controlling fluorescence intensity of a molecular probe for cellular imaging has been investigated. A reversible temperature-induced phase transition of N-isopropylacrylamide/fluorescein copolymers [poly(NIPAAm-co-FL)] was used as a molecular switch to control the fluorescence intensity of the imaging probe. The copolymer displayed environmentally sensitive fluorescence properties, in which the fluorescence intensity changed with the response to both the temperature and the pH. Utilizing these features, we monitored the thermal-aggregation process of BSA by the fluorescence resonance energy transfer (FRET) method. Additionally, the cellular uptake in RAW264.7 cells of poly(NIPAAm-co-FL) conjugated with lipid was studied using a confocal laser scanning microscope. |
| 巻・号 | 25(8) |
| ページ | 1043-7 |
| 公開日 | 2009-8-1 |
| DOI | 10.2116/analsci.25.1043 |
| PII | JST.JSTAGE/analsci/25.1043 |
| PMID | 19667485 |
| MeSH | Acrylic Resins / chemistry* Acrylic Resins / metabolism Animals Cell Line Fluoresceins / chemistry* Fluoresceins / metabolism Fluorescence Resonance Energy Transfer Hydrogen-Ion Concentration Lipids / chemistry Molecular Probes / chemistry* Molecular Probes / metabolism Nanotechnology Rats Serum Albumin, Bovine / metabolism* Temperature |
| IF | 2.049 |
| 引用数 | 14 |
| WOS 分野 | CHEMISTRY, ANALYTICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |