論文 - 詳細
| RRC ID | 37871 |
|---|---|
| 著者 | Takayuki Hoshino, Keisuke Morishima |
| タイトル | Electron-beam direct processing on living cell membrane |
| ジャーナル | Applied Physics Letters |
| Abstract |
We demonstrated a direct processing on a living Hep G2 cell membrane in conventional cultivation conditions using an electron beam. Electron beam-induced deposition from liquid precursor 3,4-ethylenedioxythiophene and ablation was performed on the living cells. The 2.5-10 keV electron beam which was irradiated through a 100-nm-thick SiN nanomembrane could induce a deposition pattern and a ablation on a living cell membrane. This electron beam direct processing can provide simple in-situ cell surface modification for an analytical method of living cell membrane dynamic. |
| 巻・号 | 99 |
| 公開日 | 2011-10-24 |
| DOI | 10.1063/1.3653278 |
| PMID | 19718512 |
| MeSH | Animals Bombyx / genetics* High-Throughput Screening Assays / methods Molecular Biology / methods Nucleopolyhedroviruses / genetics* Plasmids / genetics Recombinant Proteins / biosynthesis* Recombinant Proteins / genetics* Recombinant Proteins / isolation & purification Transfection |
| IF | 3.597 |
| 引用数 | 3 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1648) |