論文 - 詳細
RRC ID | 37871 |
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著者 | Nagaya H. |
タイトル | Recombinant protein production by a Kaiko-baculovirus system. |
ジャーナル | Methods Mol Biol |
Abstract |
Nucleopolyhedrovirus, a baculovirus, generates many intranuclear polyhedra in lepidopterous insects. The replacement of the polyhedra gene with a target gene, under a potent polyhedrin promoter, is widely used to express recombinant proteins. In this chapter, we describe the application of a highly efficient and reproducible baculovirus expression system with high throughput using Kaiko (silkworm). |
巻・号 | 577 |
ページ | 109-20 |
公開日 | 2009-1-1 |
DOI | 10.1007/978-1-60761-232-2_9 |
PMID | 19718512 |
MeSH | Animals Bombyx / genetics* High-Throughput Screening Assays / methods Molecular Biology / methods Nucleopolyhedroviruses / genetics* Plasmids / genetics Recombinant Proteins / biosynthesis* Recombinant Proteins / genetics* Recombinant Proteins / isolation & purification Transfection |
引用数 | 3 |
WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 |