RRC ID 37871
Author Takayuki Hoshino, Keisuke Morishima
Title Electron-beam direct processing on living cell membrane
Journal Applied Physics Letters
Abstract We demonstrated a direct processing on a living Hep G2 cell membrane in conventional cultivation conditions using an electron beam. Electron beam-induced deposition from liquid precursor 3,4-ethylenedioxythiophene and ablation was performed on the living cells. The 2.5-10 keV electron beam which was irradiated through a 100-nm-thick SiN nanomembrane could induce a deposition pattern and a ablation on a living cell membrane. This electron beam direct processing can provide simple in-situ cell surface modification for an analytical method of living cell membrane dynamic.
Volume 99
Published 2011-10-24
DOI 10.1063/1.3653278
PMID 19718512
MeSH Animals Bombyx / genetics* High-Throughput Screening Assays / methods Molecular Biology / methods Nucleopolyhedroviruses / genetics* Plasmids / genetics Recombinant Proteins / biosynthesis* Recombinant Proteins / genetics* Recombinant Proteins / isolation & purification Transfection
IF 3.597
Times Cited 3
WOS Category BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells Hep G2(RCB1648)