RRC ID 37871
著者 Nagaya H.
タイトル Recombinant protein production by a Kaiko-baculovirus system.
ジャーナル Methods Mol Biol
Abstract Nucleopolyhedrovirus, a baculovirus, generates many intranuclear polyhedra in lepidopterous insects. The replacement of the polyhedra gene with a target gene, under a potent polyhedrin promoter, is widely used to express recombinant proteins. In this chapter, we describe the application of a highly efficient and reproducible baculovirus expression system with high throughput using Kaiko (silkworm).
巻・号 577
ページ 109-20
公開日 2009-1-1
DOI 10.1007/978-1-60761-232-2_9
PMID 19718512
MeSH Animals Bombyx / genetics* High-Throughput Screening Assays / methods Molecular Biology / methods Nucleopolyhedroviruses / genetics* Plasmids / genetics Recombinant Proteins / biosynthesis* Recombinant Proteins / genetics* Recombinant Proteins / isolation & purification Transfection
引用数 3
WOS 分野 BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞