論文 - 詳細
| RRC ID | 37873 |
|---|---|
| 著者 | Sakurai Y, Hatakeyama H, Akita H, Oishi M, Nagasaki Y, Futaki S, Harashima H. |
| タイトル | Efficient short interference RNA delivery to tumor cells using a combination of octaarginine, GALA and tumor-specific, cleavable polyethylene glycol system. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
We recently developed a multifunctional envelope-type nano device (MEND) for efficient nucleic acid delivery. Here, we report on the development of an octaarigine (R8)-modified MEND encapsulating small interfering RNA (siRNA) with a tumor-specific, cleavable, polyethylene glycol (PEG)-lipid (PPD). We first determined the optimal concentration of R8 and pH-sensitive fusogenic peptide (GALA) on the lipid envelope of MEND (R8/GALA-MEND). Then, we examined the combination of optimized R8/GALA-MEND with a PEG-lipid. When a conventional PEG-lipid was used, the R8/GALA-MEND failed to knockdown expression of the target gene. On the other hand, PPD-modified R8/GALA-MEND exhibited efficient silencing activity to the level of the PEG-unmodified R8/GALA-MEND. In addition, we compared a R8/GALA-MEND with a MEND composed of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) that is a conventional cationic lipid used as a lipoplex component. The knockdown ability of the R8/GALA-MEND was much higher than that of the DOTAP-based MEND at the dose that is commonly employed in in vitro siRNA transfection. These results demonstrate that the R8/GALA-MEND is a promising delivery system for the transfer of siRNA to tumor cells. |
| 巻・号 | 32(5) |
| ページ | 928-32 |
| 公開日 | 2009-5-1 |
| DOI | 10.1248/bpb.32.928 |
| PII | JST.JSTAGE/bpb/32.928 |
| PMID | 19420766 |
| MeSH | Drug Delivery Systems / methods* Fatty Acids, Monounsaturated / chemistry Gene Silencing / drug effects Gene Transfer Techniques* HeLa Cells Humans Luciferases / genetics Nanoparticles / chemistry Oligopeptides / chemistry* Peptides / chemistry* Polyethylene Glycols / chemistry* Quaternary Ammonium Compounds / chemistry RNA, Small Interfering / administration & dosage* Transfection |
| IF | 1.863 |
| 引用数 | 35 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |