| RRC ID |
37874
|
| 著者 |
Takei T, Nakahara H, Ijima H, Kawakami K.
|
| タイトル |
Synthesis of a chitosan derivative soluble at neutral pH and gellable by freeze-thawing, and its application in wound care.
|
| ジャーナル |
Acta Biomater
|
| Abstract |
Conventional chitosan hydrogels exhibit an acidic nature and contain unfavorable additives because (i) chitosan is soluble only in acidic solutions and (ii) toxic chemicals or proteins of non-human origin that serve as antigens are necessary for preparing chitosan hydrogels. These characteristics of the chitosan hydrogels limit their possibilities as wound dressings. In this study, a chitosan-gluconic acid conjugate is developed, soluble in an aqueous solution at neutral pH and gellable by freeze-thawing (cryogelation) without using additives. The viability of L929 fibroblasts cultured in the presence of the chitosan derivative for 24 h was >96%. The degradation rate of the corresponding chitosan cryogels by lysozyme was tunable via the derivative concentration in the gels. The gels had low cellular adhesiveness. The gels promoted the accumulation of inflammatory cells such as polymorphonuclear leukocytes, which have the potential to release chemical mediators effective for wound healing, in full-thickness skin wounds in rats and accelerated the healing of the wounds. These results demonstrate that cryogels are promising for wound care.
|
| 巻・号 |
8(2)
|
| ページ |
686-93
|
| 公開日 |
2012-2-1
|
| DOI |
10.1016/j.actbio.2011.10.005
|
| PII |
S1742-7061(11)00436-3
|
| PMID |
22023751
|
| MeSH |
Animals
Cell Adhesion / drug effects
Cell Survival / drug effects
Cells, Cultured
Chickens
Chitosan / chemical synthesis*
Chitosan / chemistry
Chitosan / pharmacology*
Cryogels / chemistry*
Fibroblasts / cytology
Fibroblasts / drug effects
Freezing*
Gluconates / chemical synthesis
Gluconates / chemistry
Humans
Hydrogen-Ion Concentration / drug effects
Male
Mice
Muramidase / metabolism
Nephelometry and Turbidimetry
Rats
Solubility / drug effects
Solutions
Spectroscopy, Fourier Transform Infrared
Wound Healing / drug effects*
|
| IF |
7.242
|
| 引用数 |
52
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
|
