RRC ID 37876
Author Kunitomi A, Yuasa S, Sugiyama F, Saito Y, Seki T, Kusumoto D, Kashimura S, Takei M, Tohyama S, Hashimoto H, Egashira T, Tanimoto Y, Mizuno S, Tanaka S, Okuno H, Yamazawa K, Watanabe H, Oda M, Kaneda R, Matsuzaki Y, Nagai T, Okano H, Yagami KI, Tanaka M, Fukuda K.
Title H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells.
Journal Stem Cell Reports
Abstract Embryonic stem cells (ESCs) are a hallmark of ideal pluripotent stem cells. Epigenetic reprogramming of induced pluripotent stem cells (iPSCs) has not been fully accomplished. iPSC generation is similar to somatic cell nuclear transfer (SCNT) in oocytes, and this procedure can be used to generate ESCs (SCNT-ESCs), which suggests the contribution of oocyte-specific constituents. Here, we show that the mammalian oocyte-specific linker histone H1foo has beneficial effects on iPSC generation. Induction of H1foo with Oct4, Sox2, and Klf4 significantly enhanced the efficiency of iPSC generation. H1foo promoted in vitro differentiation characteristics with low heterogeneity in iPSCs. H1foo enhanced the generation of germline-competent chimeric mice from iPSCs in a manner similar to that for ESCs. These findings indicate that H1foo contributes to the generation of higher-quality iPSCs.
Volume 6(6)
Pages 825-833
Published 2016-6-14
DOI 10.1016/j.stemcr.2016.04.015
PII S2213-6711(16)30040-6
PMID 27237376
PMC PMC4912480
MeSH Animals Cellular Reprogramming* Chimerism Embryo, Mammalian Epigenesis, Genetic* Female Fibroblasts / cytology Fibroblasts / metabolism Gene Expression Histones / genetics* Histones / metabolism Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism* Kruppel-Like Factor 4 Kruppel-Like Transcription Factors / genetics Kruppel-Like Transcription Factors / metabolism Mice Mice, Transgenic Octamer Transcription Factor-3 / genetics Octamer Transcription Factor-3 / metabolism Oocytes / cytology Oocytes / metabolism* Proto-Oncogene Proteins c-myc / genetics Proto-Oncogene Proteins c-myc / metabolism SOXB1 Transcription Factors / genetics SOXB1 Transcription Factors / metabolism
IF 6.032
Times Cited 14
Human and Animal Cells B6J-23^(UTR)(AES0143) B6mt-1(AES0033)