| RRC ID |
37882
|
| Author |
Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T.
|
| Title |
TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.
|
| Journal |
Sci Rep
|
| Abstract |
The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, we show for the first time that TRPV-1 activation through transient culture at 42 °C in combination with agonists is a simple and useful strategy to eliminate iPS cells from bioengineered cardiac cell sheet tissues. When human iPS cells were cultured at 42 °C, almost all cells disappeared by 48 hours through apoptosis. However, iPS cell-derived cardiomyocytes and fibroblasts maintained transcriptional and protein expression levels, and cardiac cell sheets were fabricated after reducing the temperature. TRPV-1 expression in iPS cells was upregulated at 42 °C, and iPS cell death at 42 °C was TRPV-1-dependent. Furthermore, TRPV-1 activation through thermal or agonist treatment eliminated iPS cells in cardiac tissues for a final concentration of 0.4% iPS cell contamination. These findings suggest that the difference in tolerance to TRPV-1 activation between iPS cells and iPS cell-derived cardiac cells could be exploited to eliminate remaining iPS cells in bioengineered cell sheet tissues, which will further reduce the risk of tumour formation.
|
| Volume |
6
|
| Pages |
21747
|
| Published |
2016-2-18
|
| DOI |
10.1038/srep21747
|
| PII |
srep21747
|
| PMID |
26888607
|
| PMC |
PMC4757885
|
| MeSH |
Cell Differentiation
Cells, Cultured
Coculture Techniques
Fibroblasts / cytology
Fibroblasts / metabolism
Hot Temperature
Humans
Induced Pluripotent Stem Cells / cytology*
Myocytes, Cardiac / cytology*
Myocytes, Cardiac / metabolism
TRPV Cation Channels / agonists
TRPV Cation Channels / genetics*
TRPV Cation Channels / metabolism*
Tissue Engineering / methods*
|
| IF |
3.998
|
| Times Cited |
14
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
201B7(HPS0063)
253G1(HPS0002) |
