論文 - 詳細
| RRC ID | 37883 |
|---|---|
| 著者 | Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K. |
| タイトル | Co-ordinated ocular development from human iPS cells and recovery of corneal function. |
| ジャーナル | Nature |
| Abstract |
The eye is a complex organ with highly specialized constituent tissues derived from different primordial cell lineages. The retina, for example, develops from neuroectoderm via the optic vesicle, the corneal epithelium is descended from surface ectoderm, while the iris and collagen-rich stroma of the cornea have a neural crest origin. Recent work with pluripotent stem cells in culture has revealed a previously under-appreciated level of intrinsic cellular self-organization, with a focus on the retina and retinal cells. Moreover, we and others have demonstrated the in vitro induction of a corneal epithelial cell phenotype from pluripotent stem cells. These studies, however, have a single, tissue-specific focus and fail to reflect the complexity of whole eye development. Here we demonstrate the generation from human induced pluripotent stem cells of a self-formed ectodermal autonomous multi-zone (SEAM) of ocular cells. In some respects the concentric SEAM mimics whole-eye development because cell location within different zones is indicative of lineage, spanning the ocular surface ectoderm, lens, neuro-retina, and retinal pigment epithelium. It thus represents a promising resource for new and ongoing studies of ocular morphogenesis. The approach also has translational potential and to illustrate this we show that cells isolated from the ocular surface ectodermal zone of the SEAM can be sorted and expanded ex vivo to form a corneal epithelium that recovers function in an experimentally induced animal model of corneal blindness. |
| 巻・号 | 531(7594) |
| ページ | 376-80 |
| 公開日 | 2016-3-17 |
| DOI | 10.1038/nature17000 |
| PII | nature17000 |
| PMID | 26958835 |
| MeSH | Animals Cell Lineage Cornea / cytology* Cornea / growth & development* Cornea / physiology Corneal Transplantation Ectoderm / cytology Epithelial Cells / cytology Epithelium, Corneal / cytology Female Humans Induced Pluripotent Stem Cells / cytology* Lens, Crystalline / cytology Mice Morphogenesis Phenotype Rabbits Recovery of Function* Retinal Pigment Epithelium / cytology |
| IF | 42.779 |
| 引用数 | 67 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 279 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) 253G1(HPS0002) 454E2(HPS0077) |