論文 - 詳細
| RRC ID | 37885 |
|---|---|
| 著者 | Aini H, Itaka K, Fujisawa A, Uchida H, Uchida S, Fukushima S, Kataoka K, Saito T, Chung UI, Ohba S. |
| タイトル | Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment. |
| ジャーナル | Sci Rep |
| Abstract |
Osteoarthritis (OA) is a chronic degenerative joint disease and a major health problem in the elderly population. No disease-modifying osteoarthritis drug (DMOAD) has been made available for clinical use. Here we present a disease-modifying strategy for OA, focusing on messenger RNA (mRNA) delivery of a therapeutic transcription factor using polyethylene glycol (PEG)-polyamino acid block copolymer-based polyplex nanomicelles. When polyplex nanomicelles carrying the cartilage-anabolic, runt-related transcription factor (RUNX) 1 mRNA were injected into mouse OA knee joints, OA progression was significantly suppressed compared with the non-treatment control. Expressions of cartilage-anabolic markers and proliferation were augmented in articular chondrocytes of the RUNX1-injected knees. Thus, this study provides a proof of concept of the treatment of degenerative diseases such as OA by the in situ mRNA delivery of therapeutic transcription factors; the presented approach will directly connect basic findings on disease-protective or tissue-regenerating factors to disease treatment. |
| 巻・号 | 6 |
| ページ | 18743 |
| 公開日 | 2016-1-5 |
| DOI | 10.1038/srep18743 |
| PII | srep18743 |
| PMID | 26728350 |
| PMC | PMC4700530 |
| MeSH | Animals Cartilage, Articular / metabolism* Cartilage, Articular / pathology Disease Models, Animal Gene Expression Gene Transfer Techniques Genes, Reporter Injections, Intra-Articular Mice Micelles Nanocomposites / administration & dosage Nanocomposites / chemistry Osteoarthritis / genetics* Osteoarthritis / metabolism* Osteoarthritis / pathology Osteoarthritis / therapy Osteoarthritis, Knee / genetics Osteoarthritis, Knee / metabolism Osteoarthritis, Knee / pathology Osteoarthritis, Knee / therapy RNA, Messenger / administration & dosage* RNA, Messenger / chemistry RNA, Messenger / genetics* Transcription Factors / genetics* Transcription Factors / metabolism |
| IF | 3.998 |
| 引用数 | 38 |
| WOS 分野 | RHEUMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 27 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) 293(RCB1637) |