| RRC ID |
37896
|
| 著者 |
Itagaki T, Arima Y, Kuwabara R, Kitamura N, Iwata H.
|
| タイトル |
Interaction between cells and poly(ethylene glycol)-lipid conjugates.
|
| ジャーナル |
Colloids Surf B Biointerfaces
|
| Abstract |
Eight types of poly(ethylene glycol)-lipid(PEG-lipids) carrying different lipid tails were synthesized. These PEG-lipids were labeled with fluorescein isothiocyanate (FITC-PEG-lipids) to examine their interaction with cells and to quantitatively determine amounts of PEG-lipids bound on the cell surface. FITC-PEG-lipids spontaneously anchored to the cell membrane within 15 min without loss of cell viability. The type of lipid had very little effect on the anchoring rates, while an increase in the hydrophobicity of the lipid portion of the PEG-lipids slowed their dissociation rates. Densities of FITC-PEG-lipids on the cell surface ranged from 1 × 10(-3) to 1 × 10(-2)molecules/nm(2), depending on the kinds of lipids employed. The relationship between the stability of the lipids on the cell membrane and the hydrophobicity of the lipid moieties will give a basis for the selection of a hydrophobic moiety in PEG-lipid conjugates for use in specific applications.
|
| 巻・号 |
135
|
| ページ |
765-773
|
| 公開日 |
2015-11-1
|
| DOI |
10.1016/j.colsurfb.2015.08.014
|
| PII |
S0927-7765(15)30130-2
|
| PMID |
26342322
|
| MeSH |
Cell Line, Tumor
HEK293 Cells
Humans
Lipids / chemistry*
Polyethylene Glycols / chemistry*
|
| IF |
4.389
|
| 引用数 |
9
|
|
WOS 分野
|
BIOPHYSICS
MATERIALS SCIENCE, BIOMATERIALS
CHEMISTRY, PHYSICAL
|
| リソース情報 |
| ヒト・動物細胞 |
P3-X63-Ag8.653(RCB0146) |
