論文 - 詳細
| RRC ID | 37935 |
|---|---|
| 著者 | Nakamoto T, Izu Y, Kawasaki M, Notomi T, Hayata T, Noda M, Ezura Y. |
| タイトル | Mice Deficient in CIZ/NMP4 Develop an Attenuated Form of K/BxN-Serum Induced Arthritis. |
| ジャーナル | J Cell Biochem |
| Abstract |
CIZ/NMP4 (Cas interacting zinc finger protein, Nmp4, Zfp384) is a transcription factor that is known to regulate matrix related-proteins. To explore the possible pathophysiological role of CIZ/NMP4 in arthritis, we examined CIZ/NMP4 expression in articular cartilage in arthritis model. CIZ/NMP4 was expressed in the articular chondrocytes of mice at low levels while its expression was enhanced when arthritis was induced. Arthritis induction increased clinical score in wild type mice. In contrast, CIZ/NMP4 deficiency suppressed such rise in the levels of arthritis score and swelling of soft tissue. CIZ/NMP4 deficiency also reduced invasion of inflammatory cells in joint tissue. Quantitative PCR analyses of mRNA from joints revealed that arthritis-induced increase in expressions of IL-1β was suppressed by CIZ/NMP4 deficiency. CIZ/NMP4 bound to IL-1β promoter and activated its transcription. The increase in CIZ/NMP4 in arthritis was also associated with enhancement in bone resorption and cartilage matrix degradation. In fact, RANKL, a signaling molecule prerequisite for osteoclastogenesis and, MMP-3, a clinical marker for arthritis were increased in joints upon arthritis induction. In contrast, CIZ/NMP4 deficiency suppressed the arthritis-induced increase in bone resorption, expression of RANKL and MMP-3 mRNA. Thus, CIZ/NMP4 plays a role in the development of arthritis at least in part through regulation of key molecules related to the arthritis. |
| 巻・号 | 117(4) |
| ページ | 970-7 |
| 公開日 | 2016-4-1 |
| DOI | 10.1002/jcb.25382 |
| PMID | 26378628 |
| MeSH | Animals Arthritis, Experimental / chemically induced Arthritis, Experimental / genetics* Arthritis, Experimental / immunology Arthritis, Experimental / pathology Autoantibodies / biosynthesis Bone Resorption Cartilage, Articular / immunology* Cartilage, Articular / pathology Chondrocytes / immunology Chondrocytes / pathology Female Gene Expression Regulation Glucose-6-Phosphate Isomerase / antagonists & inhibitors Glucose-6-Phosphate Isomerase / genetics Glucose-6-Phosphate Isomerase / immunology Immune Sera / administration & dosage Interleukin-1beta / genetics Interleukin-1beta / immunology Joints / immunology Joints / pathology Male Matrix Metalloproteinase 3 / genetics Matrix Metalloproteinase 3 / immunology* Mice Mice, Knockout Nuclear Matrix-Associated Proteins / deficiency Nuclear Matrix-Associated Proteins / genetics Nuclear Matrix-Associated Proteins / immunology* Promoter Regions, Genetic RANK Ligand / genetics RANK Ligand / immunology* Severity of Illness Index Signal Transduction Transcription Factors / deficiency Transcription Factors / genetics Transcription Factors / immunology* Transcription, Genetic |
| IF | 4.237 |
| 引用数 | 4 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |