論文 - 詳細
| RRC ID | 37937 |
|---|---|
| 著者 | Fujimori T, Kato K, Fujihara S, Iwama H, Yamashita T, Kobayashi K, Kamada H, Morishita A, Kobara H, Mori H, Okano K, Suzuki Y, Masaki T. |
| タイトル | Antitumor effect of metformin on cholangiocarcinoma: In vitro and in vivo studies. |
| ジャーナル | Oncol Rep |
| Abstract |
Cholangiocarcinoma (CCA) is the most common biliary malignancy and the second most common hepatic malignancy after hepatocellular carcinoma (HCC). Treatment with the anti-diabetic drug metformin has been associated with reduced cancer incidence in patients with type 2 diabetes. Thus, the present study evaluated the effects of metformin on human CCA cell proliferation in vitro and in vivo and identified the microRNAs associated with its antitumor effects. Metformin inhibited the proliferation of the CCA cell lines HuCCT-1 and TFK-1 and blocked the G0 to G1 cell cycle transition, accompanied by AMP kinase pathway activation. Metformin treatment also led to marked decreases in cyclin D1 and cyclin-dependent kinase (Cdk) 4 protein levels and retinoblastoma protein phosphorylation. However, this drug did not affect p27kip protein expression. In addition, it reduced the phosphorylation of Axl, EphA10, ALK and PYK, as well as tumor proliferation in athymic nude mice with xenograft tumors. Furthermore, it markedly altered microRNA expression. These findings suggest that metformin may have clinical use in the treatment of CCA. |
| 巻・号 | 34(6) |
| ページ | 2987-96 |
| 公開日 | 2015-12-1 |
| DOI | 10.3892/or.2015.4284 |
| PMID | 26398221 |
| MeSH | Animals Cell Proliferation / drug effects* Cholangiocarcinoma / drug therapy* Diabetes Mellitus, Type 2 / drug therapy Gene Expression Regulation, Neoplastic / drug effects Humans Metformin / administration & dosage* Mice MicroRNAs / biosynthesis MicroRNAs / drug effects* Neoplasm Proteins / biosynthesis Xenograft Model Antitumor Assays |
| IF | 3.417 |
| 引用数 | 18 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | TFK-1(RCB2537) |