Reference - Detail
RRC ID | 37944 |
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Author | Kikuta S, Hou BH, Sato R, Frommer WB, Kikawada T. |
Title | FRET sensor-based quantification of intracellular trehalose in mammalian cells. |
Journal | Biosci Biotechnol Biochem |
Abstract |
Trehalose acts as a stress protectant and an autophagy inducer in mammalian cells. The molecular mechanisms of action remain obscure because intracellular trehalose at micromolar level is difficult to quantitate. Here, we show a novel trehalose monitoring technology based on FRET. FLIP-suc90μ∆1Venus sensor expressed in mammalian cells enables to quickly and non-destructively detect an infinitesimal amount of intracellular trehalose. |
Volume | 80(1) |
Pages | 162-5 |
Published | 2016-1-1 |
DOI | 10.1080/09168451.2015.1069699 |
PMID | 26214383 |
MeSH | Animals Bacterial Proteins / genetics* Bacterial Proteins / metabolism Biosensing Techniques* Carrier Proteins / genetics* Carrier Proteins / metabolism Cytoplasm / drug effects Cytoplasm / metabolism Diptera Escherichia coli / genetics Escherichia coli / metabolism Fluorescence Resonance Energy Transfer / methods* Gene Expression Genetic Vectors / chemistry Genetic Vectors / metabolism* HEK293 Cells Humans Insect Proteins / genetics Insect Proteins / metabolism Luminescent Proteins / genetics* Luminescent Proteins / metabolism Transfection Trehalose / analysis* Trehalose / metabolism Trehalose / pharmacology |
IF | 1.516 |
Times Cited | 5 |
WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, APPLIED |
Resource | |
Human and Animal Cells | 293T(RCB2202) |