| RRC ID |
37945
|
| Author |
Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY, Tanaka M, Miyajima A.
|
| Title |
CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.
|
| Journal |
Stem Cell Reports
|
| Abstract |
To develop a culture system for large-scale production of mature hepatocytes, liver progenitor cells (LPCs) with a high proliferation potential would be advantageous. We have found that carboxypeptidase M (CPM) is highly expressed in embryonic LPCs, hepatoblasts, while its expression is decreased along with hepatic maturation. Consistently, CPM expression was transiently induced during hepatic specification from human-induced pluripotent stem cells (hiPSCs). CPM(+) cells isolated from differentiated hiPSCs at the immature hepatocyte stage proliferated extensively in vitro and expressed a set of genes that were typical of hepatoblasts. Moreover, the CPM(+) cells exhibited a mature hepatocyte phenotype after induction of hepatic maturation and also underwent cholangiocytic differentiation in a three-dimensional culture system. These results indicated that hiPSC-derived CPM(+) cells share the characteristics of LPCs, with the potential to proliferate and differentiate bi-directionally. Thus, CPM is a useful marker for isolating hiPSC-derived LPCs, which allows development of a large-scale culture system for producing hepatocytes and cholangiocytes.
|
| Volume |
5(4)
|
| Pages |
508-15
|
| Published |
2015-10-13
|
| DOI |
10.1016/j.stemcr.2015.08.008
|
| PII |
S2213-6711(15)00242-8
|
| PMID |
26365514
|
| PMC |
PMC4624956
|
| MeSH |
Animals
Cell Culture Techniques
Cell Differentiation
Cell Line
Cell Proliferation
Cell Separation
Cells, Cultured
Embryonic Stem Cells / cytology
GPI-Linked Proteins / analysis
Hepatocytes / cytology*
Humans
Induced Pluripotent Stem Cells / cytology*
Metalloendopeptidases / analysis*
Mice
|
| IF |
6.032
|
| Times Cited |
27
|
|
WOS Category
|
CELL & TISSUE ENGINEERING
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
409B2(HPS0076)
454E2(HPS0077) |
