Reference - Detail
| RRC ID | 37945 |
|---|---|
| Author | Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY, Tanaka M, Miyajima A. |
| Title | CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells. |
| Journal | Stem Cell Reports |
| Abstract |
To develop a culture system for large-scale production of mature hepatocytes, liver progenitor cells (LPCs) with a high proliferation potential would be advantageous. We have found that carboxypeptidase M (CPM) is highly expressed in embryonic LPCs, hepatoblasts, while its expression is decreased along with hepatic maturation. Consistently, CPM expression was transiently induced during hepatic specification from human-induced pluripotent stem cells (hiPSCs). CPM(+) cells isolated from differentiated hiPSCs at the immature hepatocyte stage proliferated extensively in vitro and expressed a set of genes that were typical of hepatoblasts. Moreover, the CPM(+) cells exhibited a mature hepatocyte phenotype after induction of hepatic maturation and also underwent cholangiocytic differentiation in a three-dimensional culture system. These results indicated that hiPSC-derived CPM(+) cells share the characteristics of LPCs, with the potential to proliferate and differentiate bi-directionally. Thus, CPM is a useful marker for isolating hiPSC-derived LPCs, which allows development of a large-scale culture system for producing hepatocytes and cholangiocytes. |
| Volume | 5(4) |
| Pages | 508-15 |
| Published | 2015-10-13 |
| DOI | 10.1016/j.stemcr.2015.08.008 |
| PII | S2213-6711(15)00242-8 |
| PMID | 26365514 |
| PMC | PMC4624956 |
| MeSH | Animals Cell Culture Techniques Cell Differentiation Cell Line Cell Proliferation Cell Separation Cells, Cultured Embryonic Stem Cells / cytology GPI-Linked Proteins / analysis Hepatocytes / cytology* Humans Induced Pluripotent Stem Cells / cytology* Metalloendopeptidases / analysis* Mice |
| IF | 6.032 |
| Times Cited | 27 |
| WOS Category | CELL & TISSUE ENGINEERING CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 16 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 409B2(HPS0076) 454E2(HPS0077) |