論文 - 詳細
| RRC ID | 3800 |
|---|---|
| 著者 | Kuzuhara T, Kise D, Yoshida H, Horita T, Murazaki Y, Utsunomiya H, Tsuge H. |
| タイトル | Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2. |
| ジャーナル | Acta Crystallogr Sect F Struct Biol Cryst Commun |
| Abstract |
The C-terminal domain protein (amino-acid residues 535-759) of the PB2 subunit of the RNA-dependent RNA polymerase from the highly pathogenic influenza A virus was expressed as a soluble protein in Escherichia coli and crystallized using sodium formate as a precipitant. Data sets were collected from crystals of native and selenomethionine-substituted protein on the KEK NW12 beamline at the Photon Factory and the crystals diffracted to a maximum resolution of 2.44 A for the SeMet-derivative crystal. The native crystals were found to belong to space group P3(2)21, with unit-cell parameters a = b = 52.5, c = 156.3 A. The Matthews value (V(M)) was 2.7 A(3) Da(-1), assuming the presence of one molecule in the asymmetric unit. The SeMet-derivative crystals were found to belong to the same space group, with unit-cell parameters a = b = 52.6, c = 156.4 A. Attempts are being made to solve the structure by multi-wavelength anomalous dispersion phasing. |
| 巻・号 | 65(Pt 2) |
| ページ | 144-6 |
| 公開日 | 2009-2-1 |
| DOI | 10.1107/S1744309108042942 |
| PII | S1744309108042942 |
| PMID | 19194006 |
| PMC | PMC2635877 |
| MeSH | Binding Sites / genetics Crystallization Crystallography, X-Ray* Influenza A virus / enzymology* Influenza A virus / genetics Peptide Fragments / chemistry Peptide Fragments / genetics Peptide Fragments / metabolism Promoter Regions, Genetic* Protein Binding / genetics Protein Structure, Tertiary / genetics RNA / chemistry* RNA / genetics RNA, Viral / genetics RNA-Dependent RNA Polymerase / chemistry RNA-Dependent RNA Polymerase / genetics* RNA-Dependent RNA Polymerase / metabolism* Viral Proteins / chemistry Viral Proteins / genetics* Viral Proteins / metabolism* |
| IF | 0.968 |
| 引用数 | 5 |
| WOS 分野 | CRYSTALLOGRAPHY BIOCHEMICAL RESEARCH METHODS BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | pBMSA-PB2 (RDB01969) |