RRC ID 3800
Author Kuzuhara T, Kise D, Yoshida H, Horita T, Murazaki Y, Utsunomiya H, Tsuge H.
Title Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2.
Journal Acta Crystallogr Sect F Struct Biol Cryst Commun
Abstract The C-terminal domain protein (amino-acid residues 535-759) of the PB2 subunit of the RNA-dependent RNA polymerase from the highly pathogenic influenza A virus was expressed as a soluble protein in Escherichia coli and crystallized using sodium formate as a precipitant. Data sets were collected from crystals of native and selenomethionine-substituted protein on the KEK NW12 beamline at the Photon Factory and the crystals diffracted to a maximum resolution of 2.44 A for the SeMet-derivative crystal. The native crystals were found to belong to space group P3(2)21, with unit-cell parameters a = b = 52.5, c = 156.3 A. The Matthews value (V(M)) was 2.7 A(3) Da(-1), assuming the presence of one molecule in the asymmetric unit. The SeMet-derivative crystals were found to belong to the same space group, with unit-cell parameters a = b = 52.6, c = 156.4 A. Attempts are being made to solve the structure by multi-wavelength anomalous dispersion phasing.
Volume 65(Pt 2)
Pages 144-6
Published 2009-2-1
DOI 10.1107/S1744309108042942
PII S1744309108042942
PMID 19194006
PMC PMC2635877
MeSH Binding Sites / genetics Crystallization Crystallography, X-Ray* Influenza A virus / enzymology* Influenza A virus / genetics Peptide Fragments / chemistry Peptide Fragments / genetics Peptide Fragments / metabolism Promoter Regions, Genetic* Protein Binding / genetics Protein Structure, Tertiary / genetics RNA / chemistry* RNA / genetics RNA, Viral / genetics RNA-Dependent RNA Polymerase / chemistry RNA-Dependent RNA Polymerase / genetics* RNA-Dependent RNA Polymerase / metabolism* Viral Proteins / chemistry Viral Proteins / genetics* Viral Proteins / metabolism*
IF 0.968
Times Cited 5
DNA material pBMSA-PB2 (RDB01969)