| RRC ID |
3800
|
| 著者 |
Kuzuhara T, Kise D, Yoshida H, Horita T, Murazaki Y, Utsunomiya H, Tsuge H.
|
| タイトル |
Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2.
|
| ジャーナル |
Acta Crystallogr Sect F Struct Biol Cryst Commun
|
| Abstract |
The C-terminal domain protein (amino-acid residues 535-759) of the PB2 subunit of the RNA-dependent RNA polymerase from the highly pathogenic influenza A virus was expressed as a soluble protein in Escherichia coli and crystallized using sodium formate as a precipitant. Data sets were collected from crystals of native and selenomethionine-substituted protein on the KEK NW12 beamline at the Photon Factory and the crystals diffracted to a maximum resolution of 2.44 A for the SeMet-derivative crystal. The native crystals were found to belong to space group P3(2)21, with unit-cell parameters a = b = 52.5, c = 156.3 A. The Matthews value (V(M)) was 2.7 A(3) Da(-1), assuming the presence of one molecule in the asymmetric unit. The SeMet-derivative crystals were found to belong to the same space group, with unit-cell parameters a = b = 52.6, c = 156.4 A. Attempts are being made to solve the structure by multi-wavelength anomalous dispersion phasing.
|
| 巻・号 |
65(Pt 2)
|
| ページ |
144-6
|
| 公開日 |
2009-2-1
|
| DOI |
10.1107/S1744309108042942
|
| PII |
S1744309108042942
|
| PMID |
19194006
|
| PMC |
PMC2635877
|
| MeSH |
Binding Sites / genetics
Crystallization
Crystallography, X-Ray*
Influenza A virus / enzymology*
Influenza A virus / genetics
Peptide Fragments / chemistry
Peptide Fragments / genetics
Peptide Fragments / metabolism
Promoter Regions, Genetic*
Protein Binding / genetics
Protein Structure, Tertiary / genetics
RNA / chemistry*
RNA / genetics
RNA, Viral / genetics
RNA-Dependent RNA Polymerase / chemistry
RNA-Dependent RNA Polymerase / genetics*
RNA-Dependent RNA Polymerase / metabolism*
Viral Proteins / chemistry
Viral Proteins / genetics*
Viral Proteins / metabolism*
|
| IF |
0.968
|
| 引用数 |
5
|
|
WOS 分野
|
CRYSTALLOGRAPHY
BIOCHEMICAL RESEARCH METHODS
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pBMSA-PB2 (RDB01969) |
