論文 - 詳細
| RRC ID | 3805 |
|---|---|
| 著者 | Takeda S, Tsukiji S, Ueda H, Nagamune T. |
| タイトル | Covalent split protein fragment-DNA hybrids generated through N-terminus-specific modification of proteins by oligonucleotides. |
| ジャーナル | Org Biomol Chem |
| Abstract |
Semisynthetic protein-DNA hybrid molecules have recently attracted much attention as valuable tools for bioanalytical chemistry and nanobiotechnology. Here we describe a synthetic method for conjugating oligonucleotides to the N-terminus of recombinant proteins. Our strategy involves the conversion of amine-terminated oligonucleotides to thioester-functionalized oligonucleotides by using a bifunctional reagent bearing an N-hydroxysuccinimide ester and benzyl thioester group, followed by native chemical ligation with proteins containing an N-terminal cysteine. We applied this technique to construct split luciferase fragment-DNA hybrid systems in which the catalytic activity of split luciferase is restored by the re-assembly of each fragment through a specific DNA-protein or DNA-DNA interaction. Split protein fragment-DNA hybrids will offer new opportunities to explore the potential of protein-DNA conjugates for various applications. |
| 巻・号 | 6(12) |
| ページ | 2187-94 |
| 公開日 | 2008-6-21 |
| DOI | 10.1039/b720013g |
| PMID | 18528581 |
| MeSH | Base Sequence Chromatography, High Pressure Liquid Oligonucleotides / chemistry* Proteins / chemistry* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| IF | 3.412 |
| 引用数 | 19 |
| WOS 分野 | CHEMISTRY, ORGANIC |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | ETR103 (RDB01198) |