論文 - 詳細
| RRC ID | 38129 |
|---|---|
| 著者 | Ichida Y, Utsunomiya Y, Tomikawa J, Nakabayashi K, Sato T, Onodera M. |
| タイトル | Long time-course monitoring of ZFP809-mediated gene silencing in transgene expression driven by promoters containing MLV-derived PBS. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Expression of Moloney murine leukemia virus (MoMLV)-typed retroviral vectors is strictly suppressed in immature cells such as embryonic stem cells. The phenomenon known as gene silencing is primed by the sequence-specific binding of the zinc finger protein 809 (ZFP809) to the primer-binding site of the vectors. However, it has yet to be determined whether the ZFP809-mediated gene silencing is maintained over a long period. In this study, we established an experimental system that can monitor gene silencing during a long-term cell culture using flow cytometry technology combined with fluorescent reporters for the expression of ZFP809 and the transgene expression driven by the promoters of interest. Time-course analysis using our system revealed that ZFP809 maintains gene silencing effect even at a longtime period. Furthermore, our system was useful for the monitoring of ZFP809-mediated gene silencing regardless of the types of vectors and cell lines. |
| 巻・号 | 80(1) |
| ページ | 114-20 |
| 公開日 | 2016-1-1 |
| DOI | 10.1080/09168451.2015.1072461 |
| PMID | 26252886 |
| MeSH | Animals Binding Sites Biological Assay* Cell Line, Tumor DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Gene Silencing* Genes, Reporter Genetic Vectors / chemistry Genetic Vectors / metabolism* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HEK293 Cells Humans K562 Cells Mice Moloney murine leukemia virus / chemistry Moloney murine leukemia virus / genetics Moloney murine leukemia virus / metabolism* Promoter Regions, Genetic Protein Binding Stem Cells / metabolism* Stem Cells / pathology Time Factors |
| IF | 1.516 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | F9 |