論文 - 詳細
| RRC ID | 38152 |
|---|---|
| 著者 | Mizutani T, Kondo T, Darmanin S, Tsuda M, Tanaka S, Tobiume M, Asaka M, Ohba Y. |
| タイトル | A novel FRET-based biosensor for the measurement of BCR-ABL activity and its response to drugs in living cells. |
| ジャーナル | Clin Cancer Res |
| Abstract |
PURPOSE:To develop a novel diagnostic method for the assessment of drug efficacy in chronic myeloid leukemia (CML) patients individually, we generated a biosensor that enables the evaluation of BCR-ABL kinase activity in living cells using the principle of fluorescence resonance energy transfer (FRET). EXPERIMENTAL DESIGN:To develop FRET-based biosensors, we used CrkL, the most characteristic substrate of BCR-ABL, and designed a protein in which CrkL is sandwiched between Venus, a variant of YFP, and enhanced cyan fluorescent protein, so that CrkL intramolecular binding of the SH2 domain to phosphorylated tyrosine (Y207) increases FRET efficiency. After evaluation of the properties of this biosensor by comparison with established methods including Western blotting and flow cytometry, BCR-ABL activity and its response to drugs were examined in CML patient cells. RESULTS:After optimization, we obtained a biosensor that possesses higher sensitivity than that of established techniques with respect to measuring BCR-ABL activity and its suppression by imatinib. Thanks to its high sensitivity, this biosensor accurately gauges BCR-ABL activity in relatively small cell numbers and can also detect <1% minor drug-resistant populations within heterogeneous ones. We also noticed that this method enabled us to predict future onset of drug resistance as well as to monitor the disease status during imatinib therapy, using patient cells. CONCLUSION:In consideration of its quick and practical nature, this method is potentially a promising tool for the prediction of both current and future therapeutic responses in individual CML patients, which will be surely beneficial for both patients and clinicians. |
| 巻・号 | 16(15) |
| ページ | 3964-75 |
| 公開日 | 2010-8-1 |
| DOI | 10.1158/1078-0432.CCR-10-0548 |
| PII | 16/15/3964 |
| PMID | 20670950 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism Antineoplastic Agents / therapeutic use Benzamides Biosensing Techniques / methods* Blotting, Western Cell Separation Drug Resistance, Neoplasm / drug effects Flow Cytometry Fluorescence Resonance Energy Transfer / methods* Fusion Proteins, bcr-abl / analysis* Fusion Proteins, bcr-abl / metabolism Humans Imatinib Mesylate Leukemia, Myelogenous, Chronic, BCR-ABL Positive / diagnosis* Leukemia, Myelogenous, Chronic, BCR-ABL Positive / drug therapy Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism Luminescent Agents Nuclear Proteins / metabolism Phosphorylation Piperazines / therapeutic use Pyrimidines / therapeutic use Sensitivity and Specificity |
| IF | 10.107 |
| 引用数 | 56 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | K562 KU812(RCB0495) |