Reference - Detail
| RRC ID | 3815 |
|---|---|
| Author | Jung H, Toth PT, White FA, Miller RJ. |
| Title | Monocyte chemoattractant protein-1 functions as a neuromodulator in dorsal root ganglia neurons. |
| Journal | J Neurochem |
| Abstract |
It has previously been observed that expression of chemokine monocyte chemoattractant protein-1 (MCP-1/CC chemokine ligand 2 (CCL2)) and its receptor CC chemokine receptor 2 (CCR2) is up-regulated by dorsal root ganglion (DRG) neurons in association with rodent models of neuropathic pain. MCP-1 increases the excitability of nociceptive neurons after a peripheral nerve injury, while disruption of MCP-1/CCR2 signaling blocks the development of neuropathic pain, suggesting MCP-1 signaling is responsible for heightened pain sensitivity. To define the mechanisms of MCP-1 signaling in DRG, we studied intracellular processing, release, and receptor-mediated signaling of MCP-1 in DRG neurons. We found that in a focal demyelination model of neuropathic pain both MCP-1 and CCR2 were up-regulated by the same neurons including transient receptor potential vanilloid receptor subtype 1 (TRPV1) expressing nociceptors. MCP-1 expressed by DRG neurons was packaged into large dense-core vesicles whose release could be induced from the soma by depolarization in a Ca2+-dependent manner. Activation of CCR2 by MCP-1 could sensitize nociceptors via transactivation of transient receptor potential channels. Our results suggest that MCP-1 and CCR2, up-regulated by sensory neurons following peripheral nerve injury, might participate in neural signal processing which contributes to sustained excitability of primary afferent neurons. |
| Volume | 104(1) |
| Pages | 254-63 |
| Published | 2008-1-1 |
| DOI | 10.1111/j.1471-4159.2007.04969.x |
| PII | JNC4969 |
| PMID | 17944871 |
| PMC | PMC2186066 |
| MeSH | Animals Calcium / metabolism Capsaicin / pharmacology Cell Line, Transformed Chemokine CCL2 / metabolism Disease Models, Animal Drug Interactions Enzyme-Linked Immunosorbent Assay / methods Ganglia, Spinal / cytology* Gene Expression Regulation / physiology* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Humans Mice Mice, Transgenic Neurons / drug effects Neurons / metabolism* Polysaccharides Receptors, CCR2 / genetics Receptors, CCR2 / physiology* Sciatic Neuropathy / chemically induced Sciatic Neuropathy / metabolism Transfection / methods |
| IF | 4.066 |
| Times Cited | 151 |
| WOS Category | NEUROSCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | MSM Mouse BAC (RDB04214) MSMg01-529G05 |