| RRC ID |
38173
|
| Author |
Takayama Y, Kokuryo T, Yokoyama Y, Ito S, Nagino M, Hamaguchi M, Senga T.
|
| Title |
Silencing of Tousled-like kinase 1 sensitizes cholangiocarcinoma cells to cisplatin-induced apoptosis.
|
| Journal |
Cancer Lett
|
| Abstract |
Cholangiocarcinoma is a particularly devastating carcinoma with limited treatment options. Tousled-like kinase 1 (TLK1) is a serine/threonine protein kinase that regulates DNA replication and DNA repair pathways. Here, we show that TLK1 is abundantly expressed in cholangiocarcinoma as well as in cell lines derived from cholangiocarcinoma. Although siRNA knockdown of TLK1 did not affect the viability of cholangiocarcinoma cells, it sensitized cholangiocarcinoma cells to cisplatin-induced apoptosis. Immunofluorescence analysis of gammaH2AX foci showed that silencing of TLK1 enhanced DNA damage induced by cisplatin treatment. Our results suggest that TLK1 plays a pivotal role for the repair of cisplatin-induced DNA damage.
|
| Volume |
296(1)
|
| Pages |
27-34
|
| Published |
2010-10-1
|
| DOI |
10.1016/j.canlet.2010.03.011
|
| PII |
S0304-3835(10)00148-5
|
| PMID |
20381954
|
| MeSH |
Antimetabolites, Antineoplastic / pharmacology
Apoptosis / drug effects*
Bile Duct Neoplasms / genetics*
Bile Duct Neoplasms / pathology
Bile Ducts, Intrahepatic
Cell Line, Tumor
Cholangiocarcinoma / genetics*
Cholangiocarcinoma / pathology*
Cisplatin / pharmacology*
DNA Repair / genetics
DNA Replication / genetics
Deoxycytidine / analogs & derivatives
Deoxycytidine / pharmacology
Fibroblasts / cytology
Fibroblasts / physiology
Foreskin / cytology
Foreskin / physiology
Gemcitabine
Gene Knockdown Techniques
Gene Silencing*
Humans
In Situ Nick-End Labeling
Male
Protein Serine-Threonine Kinases / drug effects
Protein Serine-Threonine Kinases / genetics*
RNA, Small Interfering / genetics
Reverse Transcriptase Polymerase Chain Reaction
Transfection
|
| IF |
7.36
|
| Times Cited |
14
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
|
