Reference - Detail
| RRC ID | 38190 |
|---|---|
| Author | Oda Y, Yoshimura Y, Ohnishi H, Tadokoro M, Katsube Y, Sasao M, Kubo Y, Hattori K, Saito S, Horimoto K, Yuba S, Ohgushi H. |
| Title | Induction of pluripotent stem cells from human third molar mesenchymal stromal cells. |
| Journal | J Biol Chem |
| Abstract |
The expression of four transcription factors (OCT3/4, SOX2, KLF4, and MYC) can reprogram mouse as well as human somatic cells to induced pluripotent stem (iPS) cells. We generated iPS cells from mesenchymal stromal cells (MSCs) derived from human third molars (wisdom teeth) by retroviral transduction of OCT3/4, SOX2, and KLF4 without MYC, which is considered as oncogene. Interestingly, some of the clonally expanded MSCs could be used for iPS cell generation with 30-100-fold higher efficiency when compared with that of other clonally expanded MSCs and human dermal fibroblasts. Global gene expression profiles demonstrated some up-regulated genes regarding DNA repair/histone conformational change in the efficient clones, suggesting that the processes of chromatin remodeling have important roles in the cascade of iPS cells generation. The generated iPS cells resembled human embryonic stem (ES) cells in many aspects, including morphology, ES marker expression, global gene expression, epigenetic states, and the ability to differentiate into the three germ layers in vitro and in vivo. Because human third molars are discarded as clinical waste, our data indicate that clonally expanded MSCs derived from human third molars are a valuable cell source for the generation of iPS cells. |
| Volume | 285(38) |
| Pages | 29270-8 |
| Published | 2010-9-17 |
| DOI | 10.1074/jbc.M109.055889 |
| PII | S0021-9258(20)52646-X |
| PMID | 20595386 |
| PMC | PMC2937959 |
| MeSH | Animals Cell Differentiation / genetics Cell Differentiation / physiology* Cells, Cultured Humans Immunohistochemistry Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Karyotyping Kruppel-Like Factor 4 Mice Oligonucleotide Array Sequence Analysis Stromal Cells / cytology* Stromal Cells / metabolism Tooth / cytology* |
| IF | 4.238 |
| Times Cited | 89 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 13 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MC3T3-G2/PA6(RCB1127) |