論文 - 詳細
| RRC ID | 38207 |
|---|---|
| 著者 | Inami Y, Yoshikai T, Ito S, Nishio N, Suzuki H, Sakurai H, Isobe K. |
| タイトル | Differentiation of induced pluripotent stem cells to thymic epithelial cells by phenotype. |
| ジャーナル | Immunol Cell Biol |
| Abstract |
Thymic epithelial cells (TECs) are present in both cortical and medullary thymic areas, and have crucial roles in functional T-cell development. In this study, we studied the differentiation of induced pluripotent stem cells (iPSCs) to TEC. When iPSC were cultured for 4 days in collagen IV-coated dishes in the presence of both activin A and lithium chloride (LiCl), the cells differentiated to definitive endoderm through mesendoderm. Further treatment with Fgf8 followed by Fgf7, Fgf10 and BMP4 differentiated iPSC to thymic epithelial progenitor cells (TEPCs) by phenotype. Gene expression of Hoxa3, Pax1 and Pax9 was observed and cell surface proteins EpCAM1 and MTS24 were detected at day 14 of iPSC differentiation. TEPCs differentiated to medullary TECs (mTECs) by phenotype following the addition of receptor activator nuclear factor B ligand with LiCl. Thus, we successfully induced efficient differentiation from mouse iPSC to TEPCs and mTEC by phenotype using chemically defined conditions. |
| 巻・号 | 89(2) |
| ページ | 314-21 |
| 公開日 | 2011-2-1 |
| DOI | 10.1038/icb.2010.96 |
| PII | icb201096 |
| PMID | 20680027 |
| MeSH | Activins / pharmacology Animals Cell Differentiation* / drug effects Embryonic Stem Cells / cytology Embryonic Stem Cells / drug effects Embryonic Stem Cells / metabolism Endoderm / cytology Epithelial Cells / cytology* Epithelial Cells / drug effects Epithelial Cells / metabolism Gene Expression Regulation / drug effects Immunohistochemistry Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects Induced Pluripotent Stem Cells / metabolism Lithium Chloride / pharmacology Mice Phenotype RNA, Messenger / genetics RNA, Messenger / metabolism Thymus Gland / cytology* |
| IF | 3.764 |
| 引用数 | 19 |
| WOS 分野 | IMMUNOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |