| RRC ID |
38219
|
| Author |
Kawamichi Y, Cui CH, Toyoda M, Makino H, Horie A, Takahashi Y, Matsumoto K, Saito H, Ohta H, Saito K, Umezawa A.
|
| Title |
Cells of extraembryonic mesodermal origin confer human dystrophin in the mdx model of Duchenne muscular dystrophy.
|
| Journal |
J Cell Physiol
|
| Abstract |
Duchenne muscular dystrophy is an X-linked recessive genetic disease characterized by severe skeletal muscular degeneration. The placenta is considered to be a promising candidate cell source for cellular therapeutics because it contains a large number of cells and heterogenous cell populations with myogenic potentials. We analyzed the myogenic potential of cells obtained from six parts of the placenta, that is, umbilical cord, amniotic epithelium, amniotic mesoderm, chorionic plate, villous chorion, and decidua basalis. In vitro cells derived from amniotic mesoderm, chorionic plate, and villous chorion efficiently transdifferentiate into myotubes. In addition, in vivo implantation of placenta-derived cells into dystrophic muscles of immunodeficient mdx mice restored sarcolemmal expression of human dystrophin. Differential contribution to myogenesis in this study may be attributed to placental portion-dependent default cell state. Molecular taxonomic characterization of placenta-derived maternal and fetal cells in vitro will help determine the feasibility of cell-based therapy.
|
| Volume |
223(3)
|
| Pages |
695-702
|
| Published |
2010-6-1
|
| DOI |
10.1002/jcp.22076
|
| PMID |
20162617
|
| MeSH |
Animals
Biomarkers / metabolism
Cell Membrane / metabolism
Cell Shape
Cell Transplantation
Disease Models, Animal
Dystrophin / metabolism*
Extraembryonic Membranes / cytology*
Extraembryonic Membranes / transplantation*
Female
Humans
Mesoderm / cytology*
Mesoderm / transplantation*
Mice
Mice, Inbred mdx
Mice, SCID
Muscle Development
Muscle Fibers, Skeletal / metabolism
Muscle Fibers, Skeletal / pathology
Muscular Dystrophy, Duchenne / metabolism
Muscular Dystrophy, Duchenne / pathology
Muscular Dystrophy, Duchenne / therapy*
Oligonucleotide Array Sequence Analysis
Placenta / cytology
Pregnancy
|
| IF |
5.546
|
| Times Cited |
19
|
|
WOS Category
|
PHYSIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
