| RRC ID |
38226
|
| 著者 |
Saito A, Konno T, Ikake H, Kurita K, Ishihara K.
|
| タイトル |
Control of cell function on a phospholipid polymer having phenylboronic acid moiety.
|
| ジャーナル |
Biomed Mater
|
| Abstract |
We synthesized a water-insoluble phospholipid polymer bearing a phenylboronic acid moiety (PMBV), which induces cell adhesion through a specific interaction with the glycoprotein, fibronectin. Surface plasmon resonance analysis revealed that fibronectin was adsorbed on the PMBV surface. When fibroblasts were cultured on the PMBV surface, the cells adhered and proliferated normally while showing a spherical morphology. In addition, the adherent cells were able to detach after the addition of sugar molecules, which bound to phenylboronic acid through an exchange reaction. The cell cycle of adherent cells was evaluated with the embedded HeLa-Fucci cells by using a fluorescent ubiquitination-based cell cycle indicator. The cell-cycle analysis by fluorescence microscopy indicated that the adherent HeLa-Fucci cells tended to converge to the G1 phase. The differentiation of mesenchymal stem cells to chondrocytes was accelerated on PMBV in the presence of bone morphogenetic protein-2. We concluded that PMBV is a useful surface in experiments for assessing cellular function and differentiation.
|
| 巻・号 |
5(5)
|
| ページ |
054101
|
| 公開日 |
2010-10-1
|
| DOI |
10.1088/1748-6041/5/5/054101
|
| PII |
S1748-6041(10)44674-4
|
| PMID |
20876952
|
| MeSH |
Adsorption
Animals
Biocompatible Materials / chemistry
Bone Morphogenetic Protein 2 / pharmacology
Boronic Acids / chemistry*
Cell Adhesion / physiology
Cell Differentiation
Cell Physiological Phenomena*
Cells, Cultured
Chondrocytes / cytology
Fibroblasts / cytology
Fibronectins / chemistry
Fibronectins / metabolism
G1 Phase
HeLa Cells
Humans
L Cells
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / physiology
Mice
Molecular Structure
Phospholipids / chemistry*
Polymers / chemical synthesis
Polymers / chemistry*
Surface Plasmon Resonance
Surface Properties
|
| IF |
3.174
|
| 引用数 |
20
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
L929 |
