RRC ID 38226
Author Saito A, Konno T, Ikake H, Kurita K, Ishihara K.
Title Control of cell function on a phospholipid polymer having phenylboronic acid moiety.
Journal Biomed Mater
Abstract We synthesized a water-insoluble phospholipid polymer bearing a phenylboronic acid moiety (PMBV), which induces cell adhesion through a specific interaction with the glycoprotein, fibronectin. Surface plasmon resonance analysis revealed that fibronectin was adsorbed on the PMBV surface. When fibroblasts were cultured on the PMBV surface, the cells adhered and proliferated normally while showing a spherical morphology. In addition, the adherent cells were able to detach after the addition of sugar molecules, which bound to phenylboronic acid through an exchange reaction. The cell cycle of adherent cells was evaluated with the embedded HeLa-Fucci cells by using a fluorescent ubiquitination-based cell cycle indicator. The cell-cycle analysis by fluorescence microscopy indicated that the adherent HeLa-Fucci cells tended to converge to the G1 phase. The differentiation of mesenchymal stem cells to chondrocytes was accelerated on PMBV in the presence of bone morphogenetic protein-2. We concluded that PMBV is a useful surface in experiments for assessing cellular function and differentiation.
Volume 5(5)
Pages 054101
Published 2010-10-1
DOI 10.1088/1748-6041/5/5/054101
PII S1748-6041(10)44674-4
PMID 20876952
MeSH Adsorption Animals Biocompatible Materials / chemistry Bone Morphogenetic Protein 2 / pharmacology Boronic Acids / chemistry* Cell Adhesion / physiology Cell Differentiation Cell Physiological Phenomena* Cells, Cultured Chondrocytes / cytology Fibroblasts / cytology Fibronectins / chemistry Fibronectins / metabolism G1 Phase HeLa Cells Humans L Cells Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / physiology Mice Molecular Structure Phospholipids / chemistry* Polymers / chemical synthesis Polymers / chemistry* Surface Plasmon Resonance Surface Properties
IF 3.174
Times Cited 20
Human and Animal Cells L929