論文 - 詳細
| RRC ID | 38249 |
|---|---|
| 著者 | Tanabe H, Takayama I, Nishiyama T, Shimazaki M, Kii I, Li M, Amizuka N, Katsube K, Kudo A. |
| タイトル | Periostin associates with Notch1 precursor to maintain Notch1 expression under a stress condition in mouse cells. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Matricellular proteins, including periostin, modulate cell-matrix interactions and cell functions by acting outside of cells. METHODS AND FINDINGS:In this study, however, we reported that periostin physically associates with the Notch1 precursor at its EGF repeats in the inside of cells. Moreover, by using the periodontal ligament of molar from periostin-deficient adult mice (Pn-/- molar PDL), which is a constitutively mechanically stressed tissue, we found that periostin maintained the site-1 cleaved 120-kDa transmembrane domain of Notch1 (N1) level without regulating Notch1 mRNA expression. N1 maintenance in vitro was also observed under such a stress condition as heat and H(2)O(2) treatment in periostin overexpressed cells. Furthermore, we found that the expression of a downstream effector of Notch signaling, Bcl-xL was decreased in the Pn-/- molar PDL, and in the molar movement, cell death was enhanced in the pressure side of Pn-/- molar PDL. CONCLUSION:These results suggest the possibility that periostin inhibits cell death through up-regulation of Bcl-xL expression by maintaining the Notch1 protein level under the stress condition, which is caused by its physical association with the Notch1 precursor. |
| 巻・号 | 5(8) |
| ページ | e12234 |
| 公開日 | 2010-8-18 |
| DOI | 10.1371/journal.pone.0012234 |
| PII | e12234 |
| PMID | 20805882 |
| PMC | PMC2923609 |
| MeSH | Animals Cell Adhesion Molecules / deficiency Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism* Cell Line Cell Membrane / metabolism Gene Expression Regulation* Mice Molar / cytology* Molar / metabolism Molecular Weight Protein Binding Protein Precursors / chemistry Protein Precursors / metabolism* Protein Structure, Tertiary Receptor, Notch1 / chemistry Receptor, Notch1 / metabolism* Repetitive Sequences, Amino Acid Signal Transduction Stress, Mechanical* bcl-X Protein / genetics bcl-X Protein / metabolism |
| IF | 2.74 |
| 引用数 | 38 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) 293T(RCB2202) |